Publication: Genetic diversity and microevolution of Burkholderia pseudomallei in the environment.
Accepted Date
2008-01-07
Issued Date
2008-02-20
Copyright Date
2008
Resource Type
Language
eng
ISSN
1935-2735 (electronic)
1935-2727 (printed)
1935-2727 (printed)
Rights
Mahidol University
Rights Holder(s)
PubMed Central
Bibliographic Citation
Chantratita N, Wuthiekanun V, Limmathurotsakul D, Vesaratchavest M, Thanwisai A, Amornchai P, et al. Genetic diversity and microevolution of Burkholderia pseudomallei in the environment. PLoS Negl Trop Dis. 2008 Feb 20;2(2):e182.
Suggested Citation
Narisara Chantratita, นริศรา จันทราทิตย์, Vanaporn Wuthiekanun, วรรณพร วุฒิเอกอนันต์, Direk Limmathurotsakul, ดิเรก ลิ้มมธุรสกุล, Mongkol Vesaratchavest, มงคล เวสารัชเวศย์, Aunchalee Thanwisai, อัญชลี ฐานวิสัย, Premjit Amornchai, เปรมจิตร อมรชัย, Sarinna Tumapa, สรินนา ทุมาภา, Feil, Edward J., Day, Nicholas P., Peacock, Sharon J. Genetic diversity and microevolution of Burkholderia pseudomallei in the environment.. Chantratita N, Wuthiekanun V, Limmathurotsakul D, Vesaratchavest M, Thanwisai A, Amornchai P, et al. Genetic diversity and microevolution of Burkholderia pseudomallei in the environment. PLoS Negl Trop Dis. 2008 Feb 20;2(2):e182.. doi:10.1371/journal.pntd.0000182 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/770
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Title
Genetic diversity and microevolution of Burkholderia pseudomallei in the environment.
Author(s)
Narisara Chantratita
นริศรา จันทราทิตย์
Vanaporn Wuthiekanun
วรรณพร วุฒิเอกอนันต์
Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Mongkol Vesaratchavest
มงคล เวสารัชเวศย์
Aunchalee Thanwisai
อัญชลี ฐานวิสัย
Premjit Amornchai
เปรมจิตร อมรชัย
Sarinna Tumapa
สรินนา ทุมาภา
Feil, Edward J.
Day, Nicholas P.
Peacock, Sharon J.
นริศรา จันทราทิตย์
Vanaporn Wuthiekanun
วรรณพร วุฒิเอกอนันต์
Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Mongkol Vesaratchavest
มงคล เวสารัชเวศย์
Aunchalee Thanwisai
อัญชลี ฐานวิสัย
Premjit Amornchai
เปรมจิตร อมรชัย
Sarinna Tumapa
สรินนา ทุมาภา
Feil, Edward J.
Day, Nicholas P.
Peacock, Sharon J.
Corresponding Author(s)
Abstract
BACKGROUND: The soil dwelling Gram-negative pathogen Burkholderia pseudomallei is
the cause of melioidosis. The diversity and population structure of this organism
in the environment is poorly defined.
METHODS AND FINDINGS: We undertook a study of B. pseudomallei in soil sampled
from 100 equally spaced points within 237.5 m(2) of disused land in northeast
Thailand. B. pseudomallei was present on direct culture of 77/100 sampling
points. Genotyping of 200 primary plate colonies from three independent sampling
points was performed using a combination of pulsed field gel electrophoresis
(PFGE) and multilocus sequence typing (MLST). Twelve PFGE types and nine sequence
types (STs) were identified, the majority of which were present at only a single
sampling point. Two sampling points contained four STs and the third point
contained three STs. Although the distance between the three sampling points was
low (7.6, 7.9, and 13.3 meters, respectively), only two STs were present in more
than one sampling point. Each of the three samples was characterized by the
localized expansion of a single B. pseudomallei clone (corresponding to STs 185,
163, and 93). Comparison of PFGE and MLST results demonstrated that two STs
contained strains with variable PFGE banding pattern types, indicating geographic
structuring even within a single MLST-defined clone.
CONCLUSIONS: We discuss the implications of this extreme structuring of genotype
and genotypic frequency in terms of micro-evolutionary dynamics and ecology, and
how our results may inform future sampling strategies.