Publication: Genomic Investigations unmask Mycoplasma amphoriforme, a new respiratory pathogen.
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Accepted Date
2014-09-07
Issued Date
2015-02-01
Copyright Date
2014
Resource Type
Language
eng
ISSN
1058-4838 (printed)
1537-6591 (electronic)
1537-6591 (electronic)
Rights
Mahidol University
Rights Holder(s)
Clinical infectious diseases
Bibliographic Citation
Gillespie SH, Ling CL, Oravcova K, Pinheiro M, Wells L, Bryant JM, et al. Genomic Investigations unmask Mycoplasma amphoriforme, a new respiratory pathogen. Clin Infect Dis. 2015 Feb 1;60(3):381-8.
Suggested Citation
Gillespie, Stephen H., Ling, Clare L., Oravcova, Katarina, Pinheiro, Miguel, Wells, Louise, Bryant, Josephine M., McHugh, Timothy D., Bébéar, Cecile, Webster, David, Harris, Simon R., Seth-Smith, Helena M. B., Thomson, Nicholas R. Genomic Investigations unmask Mycoplasma amphoriforme, a new respiratory pathogen.. Gillespie SH, Ling CL, Oravcova K, Pinheiro M, Wells L, Bryant JM, et al. Genomic Investigations unmask Mycoplasma amphoriforme, a new respiratory pathogen. Clin Infect Dis. 2015 Feb 1;60(3):381-8.. doi:10.1093/cid/ciu820. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/839
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Title
Genomic Investigations unmask Mycoplasma amphoriforme, a new respiratory pathogen.
Corresponding Author(s)
Abstract
BACKGROUND: Mycoplasma amphoriforme has been associated with infection in
patients with primary antibody deficiency (PAD). Little is known about the
natural history of infection with this organism and its ability to be transmitted
in the community.
METHODS: The bacterial load was estimated in sequential sputum samples from 9
patients by quantitative polymerase chain reaction. The genomes of all available
isolates, originating from patients in the United Kingdom, France, and Tunisia,
were sequenced along with the type strain. Genomic data were assembled and
annotated, and a high-resolution phylogenetic tree was constructed.
RESULTS: By using high-resolution whole-genome sequencing (WGS) data, we show
that patients can be chronically infected with M. amphoriforme manifesting as a
relapsing-remitting bacterial load, interspersed by periods when the organism is
undetectable. Importantly, we demonstrate transmission of strains within a
clinical environment. Antibiotic resistance mutations accumulate in isolates
taken from patients who received multiple courses of antibiotics.
CONCLUSIONS: Mycoplasma amphoriforme isolates form a closely related species
responsible for a chronic relapsing and remitting infection in PAD patients in
the United Kingdom and from immunocompetent patients in other countries. We
provide strong evidence of transmission between patients attending the same
clinic, suggesting that screening and isolation may be necessary for susceptible
patients. This work demonstrates the critical role that WGS can play in rapidly
unraveling the biology of a novel pathogen.