Publication: Physiologic changes of urinary proteome by caffeine and excessive water intake
Issued Date
2017-06-27
Resource Type
ISSN
14374331
14346621
14346621
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2-s2.0-85020504127
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical Chemistry and Laboratory Medicine. Vol.55, No.7 (2017), 993-1002
Suggested Citation
Paleerath Peerapen, Nardtaya Ausakunpipat, Suchitra Sutthimethakorn, Siripat Aluksanasuwan, Arada Vinaiphat, Visith Thongboonkerd Physiologic changes of urinary proteome by caffeine and excessive water intake. Clinical Chemistry and Laboratory Medicine. Vol.55, No.7 (2017), 993-1002. doi:10.1515/cclm-2016-0464 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41864
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Title
Physiologic changes of urinary proteome by caffeine and excessive water intake
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Abstract
© 2017 Walter de Gruyter GmbH, Berlin/Boston. Background: Diurnal variations and physiologic changes of urinary proteome have been suggested in the urinary proteomics field. However, no clear evidence has been demonstrated. The present study thus aimed to define changes in urinary proteome by physiological stimuli, i.e. caffeine intake and excessive water drinking, both of which cause physiologic diuresis. Methods: Urine samples were collected from 30 healthy individuals under three different conditions: (i) morning void as the control; (ii) after drinking a cup of coffee; and (iii) after drinking 1 L of water within 20 min. Thereafter, differentially excreted proteins were analyzed by 2-DE proteomics approach and validated by Western blotting and ELISA. Results: Spot matching, quantitative intensity analysis, and ANOVA followed by Tukey's post-hoc multiple comparisons and the Bonferroni correction revealed significant differences in levels of five protein spots among three different conditions. These proteins were identified by quadrupole time-of-flight mass spectrometry (Q-TOF MS) and/or MS/MS analyses as kininogen 1 isoform 3, β-actin, prostaglandin D synthase (PGDS), fibrinogen α-chain and immunoglobulin light chain. Among these, the decreased level of immunoglobulin was successfully validated by Western blotting and ELISA. Conclusions: These data indicated that caffeine intake and excessive water drinking could affect urinary excretion of some proteins and may affect urinary proteome analysis.