Publication: Peripheral blood gene expression profiles in COPD subjects
Issued Date
2011-04-24
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ISSN
20439113
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2-s2.0-84863058420
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Bioinformatics. Vol.1, No.1 (2011)
Suggested Citation
Soumyaroop Bhattacharya, Shivraj Tyagi, Sorachai Srisuma, Dawn L. DeMeo, Steven D. Shapiro, Raphael Bueno, Edwin K. Silverman, John J. Reilly, Thomas J. Mariani Peripheral blood gene expression profiles in COPD subjects. Journal of Clinical Bioinformatics. Vol.1, No.1 (2011). doi:10.1186/2043-9113-1-12 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12536
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Title
Peripheral blood gene expression profiles in COPD subjects
Abstract
To identify non-invasive gene expression markers for chronic obstructive pulmonary disease (COPD), we performed genome-wide expression profiling of peripheral blood samples from 12 subjects with significant airflow obstruction and an equal number of non-obstructed controls. RNA was isolated from Peripheral Blood Mononuclear Cells (PBMCs) and gene expression was assessed using Affymetrix U133 Plus 2.0 arrays.Tests for gene expression changes that discriminate between COPD cases (FEV 1 < 70% predicted, FEV 1 /FVC < 0.7) and controls (FEV 1 > 80% predicted, FEV 1 /FVC > 0.7) were performed using Significance Analysis of Microarrays (SAM) and Bayesian Analysis of Differential Gene Expression (BADGE). Using either test at high stringency (SAM median FDR = 0 or BADGE p < 0.01) we identified differential expression for 45 known genes. Correlation of gene expression with lung function measurements (FEV 1 & FEV 1 /FVC), using both Pearson and Spearman correlation coefficients (p < 0.05), identified a set of 86 genes. A total of 16 markers showed evidence of significant correlation (p < 0.05) with quantitative traits and differential expression between cases and controls. We further compared our peripheral gene expression markers with those we previously identified from lung tissue of the same cohort. Two genes, RP9and NAPE-PLD, were identified as decreased in COPD cases compared to controls in both lung tissue and blood. These results contribute to our understanding of gene expression changes in the peripheral blood of patients with COPD and may provide insight into potential mechanisms involved in the disease. © 2011 Bhattacharya et al; licensee BioMed Central Ltd.