Publication:
Human Organic Anion Transporter 4 Is A Renal Apical Organic Anion/Dicarboxylate Exchanger in the Proximal Tubules

dc.contributor.authorSophapun Ekaratanawongen_US
dc.contributor.authorNaohiko Anzaien_US
dc.contributor.authorPromsuk Jutabhaen_US
dc.contributor.authorHiroki Miyazakien_US
dc.contributor.authorRie Noshiroen_US
dc.contributor.authorMichio Takedaen_US
dc.contributor.authorYoshikatsu Kanaien_US
dc.contributor.authorSamaisukh Sophasanen_US
dc.contributor.authorHitoshi Endouen_US
dc.contributor.otherKyorin University School of Medicineen_US
dc.contributor.otherFaculty of Medicine, Thammasat Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-07-24T03:38:18Z
dc.date.available2018-07-24T03:38:18Z
dc.date.issued2004-03-01en_US
dc.description.abstractHuman organic anion transporter OAT4 is expressed in the kidney and placenta and mediates high-affinity transport of estrone-3-sulfate (E 1S). Because a previous study demonstrated no trans-stimulatory effects by E1S, the mode of organic anion transport via OAT4 remains still unclear. In the present study, we examined the driving force of OAT4 using mouse proximal tubular cells stably expressing OAT4 (S2 OAT4). OAT4-mediated E1S uptake was inhibited by glutarate (GA) (IC 50: 1.25 mM) and [14C]GA uptake via S2 OAT4 was significantly trans-stimulated by unlabeled GA (5 mM) (P<0.001). [ 3H]E1S uptake via S2 OAT4 was significantly trans-stimulated by preloaded GA (P<0.001) and its [14C]GA efflux was significantly trans-stimulated by unlabeled E1S in the medium (P<0.05). In additon, both the uptake and efflux of [ 14C]p-aminohippuric acid (PAH) and [14C]GA via S 2 OAT4 were significantly trans-stimulated by unlabeled GA or PAH. The immunoreactivities of OAT4 were observed in the apical membrane of proximal tubules along with those of basolateral organic anion/dicarboxylate exchangers such as hOAT1 and hOAT3 in the same tubular population. These results indicate that OAT4 is an apical organic anion/dicarboxylate exchanger and mainly functions as an apical pathway for the reabsorption of some organic anions in renal proximal tubules driven by an outwardly directed dicarboxylate gradient.en_US
dc.identifier.citationJournal of Pharmacological Sciences. Vol.94, No.3 (2004), 297-304en_US
dc.identifier.doi10.1254/jphs.94.297en_US
dc.identifier.issn13478613en_US
dc.identifier.other2-s2.0-1842732504en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/21220
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1842732504&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleHuman Organic Anion Transporter 4 Is A Renal Apical Organic Anion/Dicarboxylate Exchanger in the Proximal Tubulesen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1842732504&origin=inwarden_US

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