Publication: Biochemical characterization of the α-Amylase inhibitor in mungbeans and its application in inhibiting the growth of callosobruchus maculatus
Issued Date
2010-02-24
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ISSN
15205118
00218561
00218561
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2-s2.0-77249141568
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Agricultural and Food Chemistry. Vol.58, No.4 (2010), 2131-2137
Suggested Citation
Anussorn Wisessing, Arunee Engkagul, Arunee Wongpiyasatid, Klattawee Choowongkomon Biochemical characterization of the α-Amylase inhibitor in mungbeans and its application in inhibiting the growth of callosobruchus maculatus. Journal of Agricultural and Food Chemistry. Vol.58, No.4 (2010), 2131-2137. doi:10.1021/jf903411x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28530
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Title
Biochemical characterization of the α-Amylase inhibitor in mungbeans and its application in inhibiting the growth of callosobruchus maculatus
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Abstract
The insect Callosobruchus maculatus causes considerable damage to harvested mungbean seeds every year, which leads to commercial losses. However, recent studies have revealed that mungbean seeds contain α-amylase inhibitors that can inhibit the protein C. maculatus, preventing growth and development of the insect larvae in the seed, thus preventing further damage. For this reason, the use of α-amylase inhibitors to interfere with the pest's digestion process has become an interesting alternative biocontrolling agent. In this study, we have isolated and purified the a-amylase inhibitor from mungbean seeds (KPSl) using ammonium sulfate precipitation, gel filtration chromatography and reversed phase HPLC. We found that the αa-amylase inhibitor, isolated as a monomer, had a molecular weight of 27 kDa. The α-amylase inhibitor was purified 750-fold with a final yield of 0.4 mg of protein per 30 g of mungbean seeds. Its specific activity was determined at 14.5 U (mg of protein) -1 . Interestingly, we found that the isolated α-amylase inhibitor inhibits C. maculatus a-amylase but not human salivary α-amylase. After preincubation of the enzyme with the inhibitor, the mungbean α-amylase inhibitor inhibited C. maculatus α-amylase activity by decreasing Vmaxwhile increasing the Km constant, indicating that the mungbean α-amylase is a mix noncompetitive inhibitor. The in vivo effect of α-amylase inhibitor on the mortality of C. maculatus shows that the α-amylase inhibitor acts on C. maculatus during the development stage, by reducing carbohydrate digestion necessary for growth and development, rather than during the end laying/ hatching stage. Our results suggest that mungbean α-amylase inhibitor could be a useful future biocontrolling agent. © 2010 American Chemical Society.