Publication: A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway
Issued Date
2020-09-01
Resource Type
ISSN
19506007
07533322
07533322
Other identifier(s)
2-s2.0-85086940608
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Mahidol University
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SCOPUS
Bibliographic Citation
Biomedicine and Pharmacotherapy. Vol.129, (2020)
Suggested Citation
Preedajit Wongkrasant, Pawin Pongkorpsakol, Jutharat Ariyadamrongkwan, Roojanaat Meesomboon, Saravut Satitsri, Rath Pichyangkura, Kim E. Barrett, Chatchai Muanprasat A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway. Biomedicine and Pharmacotherapy. Vol.129, (2020). doi:10.1016/j.biopha.2020.110415 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/58358
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Title
A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway
Abstract
© 2020 The Authors Tight junctions play an important role in maintaining barrier integrity of intestinal epithelia. Activation of AMP-activated protein kinase (AMPK) promotes tight junction assembly in intestinal epithelial cells (IEC). Fructo-oligosaccharides (FOS), well-known prebiotics, have previously been shown to alleviate inflammation-associated intestinal epithelial disruption although the mechanisms were unclear. This study aimed to investigate any effect of FOS on AMPK activity and tight junction assembly under non-inflammatory and inflammatory conditions using T84 cells as an IEC model. As analyzed by western blot, FOS induced AMPK activation through a calcium sensing receptor (CaSR)-phospholipase C (PLC)- Ca2+/calmodulin-dependent protein kinase kinase-β (CaMKKβ) pathway. Calcium switch assays and immunofluorescence staining of zonula occludens-1 (ZO-1) revealed that FOS induced tight junction assembly via an CaMKKβ-AMPK-dependent mechanism in IEC. Interestingly, FOS reversed the suppressive effect of lipopolysaccharide (LPS) on AMPK activity and tight junction assembly via a CaMKKβ pathway. Taken together, these findings uncover a prebiotic-independent effect of FOS in promoting intestinal epithelial tight junction assembly through AMPK activation, which may have implications for the treatment of diseases whose pathogenesis involves impaired intestinal barrier function.