Publication: Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application
Issued Date
1996-06-01
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-0030418721
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.14, No.1 (1996), 43-47
Suggested Citation
Supinya Pongsunk, Pattama Ekpo, Tararaj Dharakul Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application. Asian Pacific Journal of Allergy and Immunology. Vol.14, No.1 (1996), 43-47. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17742
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Title
Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application
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Abstract
Hybridomas secreting monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei were produced by immunizing BALB/cJ mice with crude culture filtrate of B. pseudomallei. Two monoclonal antibodies were found to be highly specific to B. pseudomallei as tested by indirect enzyme- linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from B. pseudomallei, B. cepacia, Pseudomonas aeruginosa, P. putida, and Escherichia coil. One of the specific MAbs, clone SP- M, IgM subclass, could directly agglutinate all 42 B. pseudomallei, isolates. The study has shown that the agglutinating MAb has potential for rapid identification of B. pseudomallei in primary bacterial culture from clinical specimens. The antibody can be used in bacteriology laboratories to reduce time of biochemical methods, which require a few days.