Journal of Fermentation and Bioengineering. Vol.86, No.3 (1998), 335-337
Suggested Citation
Saovanee Dharmsthiti, Sudaporn Luchai Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene. Journal of Fermentation and Bioengineering. Vol.86, No.3 (1998), 335-337. doi:10.1016/S0922-338X(98)80140-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18313
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Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene
Aeromonas sobria LP004 harboring the Acinetobacter calcoaceticus lipase gene was designated as strain LP094. Lipase production of LP094 in WYGS medium [Lotrakul and Dharmsthiti, World J. Microbiol. Biotechnol., 13:163-166, 1997] was scaled up to a 50-l volume. LP094 lipase immobilized by ionic binding to either IR120 (Na+) or IRC50 (H+) Amberlite resins was found to retain more than 90% activity after 15 d storage at room temperature (≈25 to 30°C) or at 4°C. After 5 repeated lipid hydrolysis reactions, the activities of both immobilized preparations remained higher than 65%.
