Publication: Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury
Issued Date
2017
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
BMC Complementary and Alternative Medicine. Vol.17, No. 1 (2017), 117
Suggested Citation
Sirintorn Srimachai, Devaux, Sylvie, Demougeot, Celine, Sarawut Kumphune, Ullrich, Nina D., Niggli, Ernst, Kornkanok Ingkaninan, Natakorn Kamkaew, Scholfield, Norman, Sompol Tapechum, Krongkarn Chootip Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury. BMC Complementary and Alternative Medicine. Vol.17, No. 1 (2017), 117. doi:DOI 10.1186/s12906-017-1637-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43718
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Title
Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury
Other Contributor(s)
Mahidol University. Faculty of Medicine, Siriraj Hospital, Department of Physiology
Naresuan University. Faculty of Medical Science. Department of Physiology
Naresuan University. Faculty of Allied Health Sciences. Department of Medical Technology
Naresuan University. Faculty of Pharmaceutical Sciences. Department of Pharmaceutical Chemistry and Pharmacognosy
University of Phayao. School of Medical Sciences
Heidelberg University. Department of Physiology and Pathophysiology
Naresuan University. Faculty of Medical Science. Department of Physiology
Naresuan University. Faculty of Allied Health Sciences. Department of Medical Technology
Naresuan University. Faculty of Pharmaceutical Sciences. Department of Pharmaceutical Chemistry and Pharmacognosy
University of Phayao. School of Medical Sciences
Heidelberg University. Department of Physiology and Pathophysiology
Abstract
Background: This study explored Bacopa monnieri, a medicinal Ayurvedic herb, as a cardioprotectant against
ischemia/reperfusion injury using cardiac function and coronary flow as end-points.
Methods: In normal isolated rat hearts, coronary flow, left ventricular developed pressure, heart rate, and functional
recovery were measured using the Langendorff preparation. Hearts were perfused with either (i) Krebs-Henseleit
(normal) solution, (control), or with 30, 100 μg/ml B. monnieri ethanolic extract (30 min), or (ii) with normal solution
or extract for 10 min preceding no-perfusion ischemia (30 min) followed by reperfusion (30 min) with
normal solution. Infarct volumes were measured by triphenyltetrazolium staining. L-type Ca2+-currents (ICa, L)
were measured by whole-cell patching in HL-1 cells, a mouse atrial cardiomyocyte cell line. Cytotoxicity of
B. monnieri was assessed in rat isolated ventricular myocytes by trypan blue exclusion.
Results: In normally perfused hearts, B. monnieri increased coronary flow by 63 ± 13% (30 μg/ml) and 216 ± 21%
(100 μg/ml), compared to control (5 ± 3%) (n = 8–10, p < 0.001). B. monnieri treatment preceding ischemia/reperfusion
improved left ventricular developed pressure by 84 ± 10% (30 μg/ml), 82 ± 10% (100 μg/ml) and 52 ± 6% (control)
compared to pre- ischemia/reperfusion. Similarly, functional recovery showed a sustained increase. Moreover, B.
monnieri (100 μg/ml) reduced the percentage of infarct size from 51 ± 2% (control) to 25 ± 2% (n = 6-8, p < 0.0001).
B. monnieri (100 μg/ml) reduced ICa, L by 63 ± 4% in HL-1 cells. Ventricular myocyte survival decreased at higher
concentrations (50–1000 μg/ml) B. monnieri.
Conclusions: B. monnieri improves myocardial function following ischemia/reperfusion injury through recovery of
coronary blood flow, contractile force and decrease in infarct size. Thus this may lead to a novel cardioprotectant
strategy.