Publication: Development and evaluation of PCR methods based on cytochrome c oxidase subunit one (cox1) and NADH dehydrogenase subunit one gene (nad1) to detect Opisthorchis viverrini in human fecal samples
Issued Date
2015-09-18
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ISSN
14321955
09320113
09320113
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2-s2.0-84939254766
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology Research. Vol.114, No.9 (2015), 3547-3549
Suggested Citation
Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Tawee Naaglor, Paanjit Taamasri, Picha Suwannahitatorn, Peerapan Tan-ariya Development and evaluation of PCR methods based on cytochrome c oxidase subunit one (cox1) and NADH dehydrogenase subunit one gene (nad1) to detect Opisthorchis viverrini in human fecal samples. Parasitology Research. Vol.114, No.9 (2015), 3547-3549. doi:10.1007/s00436-015-4640-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35096
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Title
Development and evaluation of PCR methods based on cytochrome c oxidase subunit one (cox1) and NADH dehydrogenase subunit one gene (nad1) to detect Opisthorchis viverrini in human fecal samples
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Abstract
© 2015, Springer-Verlag Berlin Heidelberg. Opisthorchis viverrini is highly prevalent throughout Southeast Asia. Chronic infection of this parasite leads to cholangiocarcinoma (CCA), a fatal bile duct cancer. The early and accurate detection of this parasite is very important; therefore, new PCR methods targeting cytochrome c oxidase subunit one and NADH dehydrogenase subunit one gene to detect O. viverrini in fecal specimens have been developed. Ninety O. viverrini-positive human fecal samples were used in this study. The PCR sensitivity of both genes was compared with internal transcribe spacer 2 (ITS2)-PCR. The sensitivity of cox1-PCR and nad1-PCR was 66.7 and 50 %, respectively. The sensitivity of cox1-PCR and nad1-PCR achieved 89.1 and 71.7 % in specimens containing O. viverrini eggs of >100 eggs per gram (EPG). Additionally, these primers can be used to provide the information on genetic diversity from mitochondrial genes of O. viverrini.