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Development and Validation of Liquid Chromatography/Tandem Mass Spectrometry Analysis for Therapeutic Drug Monitoring of Risperidone and 9-Hydroxyrisperidone in Pediatric Patients with Autism Spectrum Disorders

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dc.contributor.authorNatchaya Vanwongen_US
dc.contributor.authorSantirat Prommasen_US
dc.contributor.authorApichaya Puangpetchen_US
dc.contributor.authorYaowaluck Hongkaewen_US
dc.contributor.authorNopadol Nuntamoolen_US
dc.contributor.authorChalitpol Na Nakornen_US
dc.contributor.authorNattawat Ngamsamuten_US
dc.contributor.authorPenkhae Limsilaen_US
dc.contributor.authorChonlaphat Sukasemen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherFaculty of Medicine, Ramathibodi Hospital, Mahidol Universityen_US
dc.contributor.otherThailand Ministry of Public Healthen_US
dc.date.accessioned2018-12-11T02:07:22Z
dc.date.accessioned2019-03-14T08:03:47Z
dc.date.available2018-12-11T02:07:22Z
dc.date.available2019-03-14T08:03:47Z
dc.date.issued2016-11-01en_US
dc.description.abstract© 2016 Wiley Periodicals, Inc. Background: Risperidone (RIS) is a widely used atypical antipsychotic drug. We developed and validated a sensitive and accurate LC-MS/MS method, which requires a small-volume of plasma and small-volume injection for measurement of RIS levels in ASD pediatric patients. We also investigated the relationship between RIS levels and RIS dosages, including prolactin levels. Method: Blood samples were processed by protein precipitation extraction. Only 1 μl of sample was injected. Plasma samples were separated on a C18 column (4.6 cm × 50 mm; 1.8 μm particle size). Detection was by MS-MS with an analytical run time of 6 min. Results: The inter-day accuracy of RIS was 101.33–107.68% and 95.24–103.67% for 9-OH-RIS. The inter-day precision of RIS was ≤7.27% CV and ≤7.41% CV for 9-OH-RIS. The extraction recovery of RIS and 9-OH-RIS were 95.01 ± 7.31–112.62 ± 7.50% and 90.27 ± 11.15–114.00 ± 10.35%, respectively. This method was applied in the therapeutic drug monitoring of ASD pediatric patients. Higher RIS dosage has a tendency to produce higher RIS plasma levels. The high RIS plasma levels have a tendency to produce hyperprolactinemia. Conclusion: The determination of RIS in individual patients might be clinically useful for monitoring and prediction of treatment response.en_US
dc.identifier.citationJournal of Clinical Laboratory Analysis. Vol.30, No.6 (2016), 1236-1246en_US
dc.identifier.doi10.1002/jcla.22009en_US
dc.identifier.issn10982825en_US
dc.identifier.issn08878013en_US
dc.identifier.other2-s2.0-84977588619en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14594/42758
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977588619&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectHealth Professionsen_US
dc.subjectMedicineen_US
dc.titleDevelopment and Validation of Liquid Chromatography/Tandem Mass Spectrometry Analysis for Therapeutic Drug Monitoring of Risperidone and 9-Hydroxyrisperidone in Pediatric Patients with Autism Spectrum Disordersen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977588619&origin=inwarden_US
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