Publication: Angiotensin-converting enzyme inhibitory and antioxidant peptides from digestion of larvae and pupae of Asian weaver ant, Oecophylla smaragdina, Fabricius
Issued Date
2017-08-15
Resource Type
ISSN
10970010
00225142
00225142
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2-s2.0-85020455640
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Science of Food and Agriculture. Vol.97, No.10 (2017), 3133-3140
Suggested Citation
Werawich Pattarayingsakul, Arjaree Nilavongse, Onrapak Reamtong, Pamorn Chittavanich, Idsada Mungsantisuk, Yuvadee Mathong, Wipoo Prasitwuttisak, Watanalai Panbangred Angiotensin-converting enzyme inhibitory and antioxidant peptides from digestion of larvae and pupae of Asian weaver ant, Oecophylla smaragdina, Fabricius. Journal of the Science of Food and Agriculture. Vol.97, No.10 (2017), 3133-3140. doi:10.1002/jsfa.8155 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41405
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Title
Angiotensin-converting enzyme inhibitory and antioxidant peptides from digestion of larvae and pupae of Asian weaver ant, Oecophylla smaragdina, Fabricius
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Abstract
© 2016 Society of Chemical Industry BACKGROUND: Mixed larvae and pupae of weaver ant (Oecophylla smaragdina) are widely used as an important food ingredient in regions of Thailand. They have high nutritional values and comprise 53% protein and 13% lipid. Peptides derived from food proteins have been shown to possess biological activities. RESULTS: Peptides derived from pepsin and trypsin digestion of these weaver ant larvae and pupae were purified based on angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities, and their amino acid sequences were identified by liquid chromatography–tandem mass spectrometry (LC-MS/MS). In silico docking of peptides with ACE successfully predicted the inhibitory peptides as confirmed by their chemical synthesis. Two peptides with sequences of FFGT and LSRVP showed IC50 values for ACE inhibition of 19.5 ± 1.7 and 52.7 ± 4.0 µmol L−1, respectively. In addition, one potent antioxidant peptide with a sequence of CTKKHKPNC showed IC50 values of 48.2 ± 2.1 µmol L−1 for DPPH assay and 38.4 ± 0.2 µmol L−1 for ABTS assay, respectively. CONCLUSION: These results indicate that proteins from larvae and pupae of weaver ants are potential sources of peptides with anti-ACE and antioxidation bioactivities. © 2016 Society of Chemical Industry.