Publication:
Process optimization for an industrial-scale production of Diphtheria toxin by Corynebacterium diphtheriae PW8

dc.contributor.authorMaethichai Suwanpatcharakulen_US
dc.contributor.authorChompunut Pakdeecharoenen_US
dc.contributor.authorSupitcha Visuttitewinen_US
dc.contributor.authorNorapath Pesirikanen_US
dc.contributor.authorSomchai Chauvatcharinen_US
dc.contributor.authorThunyarat Pongtharangkulen_US
dc.contributor.otherThailand Government Pharmaceutical Organizationen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-11T02:06:57Z
dc.date.accessioned2019-03-14T08:03:33Z
dc.date.available2018-12-11T02:06:57Z
dc.date.available2019-03-14T08:03:33Z
dc.date.issued2016-11-01en_US
dc.description.abstract© 2016 International Alliance for Biological Standardization In this study, several parameters affecting the toxin production of Corynebacterium diphtheriae Parke Williams 8 (PW8) were investigated in detail. The comparison studies of amino acid profile in NZ Amine A-based medium (NZ medium) and beef digest-based medium (BD medium) suggested that an insufficient supply of amino acids was not responsible for low toxin yield observed in NZ medium. Supplementation of additional amino acids and growth promoting nutrient (in a form of yeast extract) into NZ medium enhanced only cell growth but not toxin production. Thus, BD medium was selected as the most suitable base medium for toxin production as it gave a significantly higher limit of flocculation (93 ± 0 Lf/ml) than NZ medium (46 ± 0 Lf/ml). Interestingly, a supplementation of 0.2% YE into BD medium resulted in a significant increase in growth as well as toxin production (235 ± 5 Lf/ml). In conclusion, consistently high toxin titer (174–239 Lf/ml) could be obtained from BD medium at a 5 L-scale production as long as 1) the protein content of BD medium was at least 24 g/L, 2) the iron content was below 0.15 ppm and 3) 0.2% YE was supplemented into the medium.en_US
dc.identifier.citationBiologicals. Vol.44, No.6 (2016), 534-539en_US
dc.identifier.doi10.1016/j.biologicals.2016.08.002en_US
dc.identifier.issn10958320en_US
dc.identifier.issn10451056en_US
dc.identifier.other2-s2.0-84994710624en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/42514
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994710624&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemical Engineeringen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleProcess optimization for an industrial-scale production of Diphtheria toxin by Corynebacterium diphtheriae PW8en_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994710624&origin=inwarden_US
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