Publication: Identification of dengue virus binding proteins using affinity chromatography
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Issued Date
2008-08-01
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ISSN
01660934
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2-s2.0-46949086037
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.151, No.2 (2008), 325-328
Suggested Citation
Supranee Upanan, Atichat Kuadkitkan, Duncan R. Smith Identification of dengue virus binding proteins using affinity chromatography. Journal of Virological Methods. Vol.151, No.2 (2008), 325-328. doi:10.1016/j.jviromet.2008.05.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/19302
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Title
Identification of dengue virus binding proteins using affinity chromatography
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Abstract
Several studies have identified putative dengue virus receptors using virus overlay protein binding assays (VOPBA) with some apparent success. Given that this technique relies upon the use of electrophoresis of proteins through polyacrylamide gels with varying amounts of protein denaturation, the physiological relevance of the proteins isolated is open to question. To address this issue a Sepharose 4B-dengue virus serotype 2-affinity column was constructed to selectively bind dengue virus binding proteins from HepG2 (liver) cell membrane preparations. Results show that GRP78, but not the 37/67 kDa high affinity laminin receptor, was specifically bound by the column. This result is consistent with earlier work and shows that while affinity chromatography may provide a useful adjunct to VOPBA based studies particularly in cases where proteins maybe sensitive to denaturation, proteins isolated by VOPBA can be physiologically relevant. © 2008 Elsevier B.V. All rights reserved.