Publication: Survival of Burkholderia pseudomallei in distilled water for 16 years
Issued Date
2011-10-01
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ISSN
18783503
00359203
00359203
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2-s2.0-80052967899
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.10 (2011), 598-600
Suggested Citation
Apinya Pumpuang, Narisara Chantratita, Chanthiwa Wikraiphat, Natnaree Saiprom, Nicholas P J Day, Sharon J. Peacock, Vanaporn Wuthiekanun Survival of Burkholderia pseudomallei in distilled water for 16 years. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.10 (2011), 598-600. doi:10.1016/j.trstmh.2011.06.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11981
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Title
Survival of Burkholderia pseudomallei in distilled water for 16 years
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Abstract
Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 7 cells/ml and 1.4×10 5 cells/ml, respectively. A colony count on agar of 1.0×10 6 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement. © 2011 Royal Society of Tropical Medicine and Hygiene.