The Southeast Asian journal of tropical medicine and public health. Vol.20, No.4 (1989), 519-522
Suggested Citation
V. Boonsaeng, K. Chansiri, P. Vilasineekul, P. Wilairat, S. Panyim Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.. The Southeast Asian journal of tropical medicine and public health. Vol.20, No.4 (1989), 519-522. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15783
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Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
