Publication:
Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.

1

Issued Date

1989-12-01

Resource Type

Article

ISSN

01251562

Other identifier(s)

2-s2.0-0024797308

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

The Southeast Asian journal of tropical medicine and public health. Vol.20, No.4 (1989), 519-522

Suggested Citation

V. Boonsaeng, K. Chansiri, P. Vilasineekul, P. Wilairat, S. Panyim Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.. The Southeast Asian journal of tropical medicine and public health. Vol.20, No.4 (1989), 519-522. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15783

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Title

Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.

Author(s)

V. Boonsaeng
 K. Chansiri
 P. Vilasineekul
 P. Wilairat
 S. Panyim

Other Contributor(s)

Mahidol University

Abstract

A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/15783

Collections

Scopus 1969-1990