Publication: Syringe-push membrane absorption as a simple rapid method of urine preparation for clinical proteomics
Issued Date
2015
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Language
eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Clinical Proteomics. Vol. 12, (2015), 15
Suggested Citation
Somchai Chutipongtanate, Channarong Changtong, Churat Weeraphan, Suradej Hongeng, Chantragan Srisomsap, Jisnuson Svasti Syringe-push membrane absorption as a simple rapid method of urine preparation for clinical proteomics. Clinical Proteomics. Vol. 12, (2015), 15. doi:10.1186/s12014-015-9087-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2716
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Title
Syringe-push membrane absorption as a simple rapid method of urine preparation for clinical proteomics
Abstract
Background: The analysis of urinary proteome might reveal biomarkers of clinical value. However, current methods
of urine preparation for down-stream proteomic analysis are complicated, time-consuming, and/or expensive. This
study aims to develop a robust, simple, inexpensive and readily accessible urine preparation method to facilitate
clinical proteomic workflow.
Result: Syringe-push membrane absorption (SPMA) was successfully developed by a combination of 5-ml medical
syringe and protein-absorbable membrane. Comparing three membranes i.e., nitrocellulose, polyvinylidene difluoride
(PVDF) and Whatman no.1, nitrocellulose combined with SPMA (nitrocellulose-SPMA) provided the greatest quality of
proteome profile as demonstrated by 2-DE. The quality of the proteome profile and the performance of nitrocellulose-
SPMA were systematically compared with three current methods of urine preparation (i.e., ultrafiltration, dialysis/
lyophilization and precipitation). While different methods of urine preparation provided comparable proteome quality,
nitrocellulose-SPMA had better working performance due to acceptable recovery yield, less workload, short working
time, high accessibility and low unit cost. In addition, protein absorbed on nitrocellulose harvested from the SPMA
procedure could be stored as a dried membrane at room temperature for at least 1-month without protein degradation
or modification.
Conclusions: SPMA is a simple rapid method of preparing urine for downstream proteomic analysis. Because of it is
highly accessible and has long storage duration, this technique holds potential benefit for large-scale multi-center
research and future development of clinical investigation based upon urinary proteomic analysis.