Publication: Bead array for Listeria monocytogenes detection using specific monoclonal antibodies
Issued Date
2015-01-01
Resource Type
ISSN
09567135
Other identifier(s)
2-s2.0-84907164516
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Food Control. Vol.47, (2015), 462-471
Suggested Citation
Nitsara Karoonuthaisiri, Ratthaphol Charlermroj, Jarinthorn Teerapornpuntakit, Mallika Kumpoosiri, Orawan Himananto, Irene R. Grant, Oraprapai Gajanandana, Christopher T. Elliott Bead array for Listeria monocytogenes detection using specific monoclonal antibodies. Food Control. Vol.47, (2015), 462-471. doi:10.1016/j.foodcont.2014.07.049 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35259
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Bead array for Listeria monocytogenes detection using specific monoclonal antibodies
Abstract
To develop a detection method for human pathogenic Listeria monocytogenes, novel specific antibodies were obtained from hybridoma libraries generated by using formalin-killed and heat-killed L.monocytogenes as immunogens. Several monoclonal antibodies found to be specific to Listeria spp. or L.monocytogenes were evaluated for their applicability as binders for bead array and sandwich ELISA for detection of L.monocytogenes in buffer and in 11 different food types. The bead array format consistently demonstrated lower detection limits and was less affected by interference from food matrices than the sandwich ELISA format. However, the obtained detection limits were not sufficient to satisfy the required standard for L.monocytogenes testing. Therefore, the international organization for standardization (ISO 11290-1:1996) methods for pre-enrichment and enrichment were employed to increase the bacteria numbers. When compared to the standard plating method, the bead array was able to detect the bacteria with the same accuracy even at the 1CFU level after only 24h of the enrichment period. In addition, Listeria-specific 3C3 and L.monocytogenes-specific 7G4 antibodies were successfully employed to construct a multiplex detection for Listeria, Salmonella and Campylobacter in a bead array format by combining with commercial Salmonella-specific and available Campylobacter-specific antibodies. © 2014 Elsevier Ltd.