Use of buffy coat thick films in detecting malaria parasites in patients with negative conventional thick films

Abstract

Objective: To determine the frequency of malaria parasite detection from the buffy coat bloodfilms by using capillary tube in falciparum malaria patients with negative conventional thickfilms. Methods: Thirty six uncomplicated falciparum malaria patients confirmed by conventionalthick and thin films were included in the study. The patients were treated with artemisinin combination therapy at Hospital for Tropical Diseases, Bangkok, Thailand for 28 day. Fingerpricks for conventional blood films were conducted every 6 hours until negative parasitemia, then dailyfingerpricks for parasite checks were conducted until the patients were discharged from hospital. Blood samples were also concurrently collected in 3 heparinized capillary tubes at the same timeof fingerpricks for conventional blood films when the prior parasitemia was negative on thin filmsand parasitemia was lower than 50 parasites/200 white blood cells by thick film. The first negativeconventional thick films were compared with buffy coat thick films for parasite identification.Results: Out of 36 patients with thick films showing negative for asexual forms of parasites, buffy coat films could detect remaining 10 patients (27.8%) with asexual forms of Plasmodium falciparum. Conclusions: The study shows that buffy coat thick films are useful and can detect malarial parasites in 27.8% of patients whose conventional thick films show negative parasitemia.