Publication: Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine
Issued Date
2021-01-01
Resource Type
ISSN
15324117
10934529
10934529
Other identifier(s)
2-s2.0-85106517702
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. Vol.56, No.7 (2021), 769-785
Suggested Citation
Prinpida Sonthiphand, Supeerapat Kraidech, Saowarod Polart, Srilert Chotpantarat, Kanthida Kusonmano, Pichahpuk Uthaipaisanwong, Chalida Rangsiwutisak, Ekawan Luepromchai Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine. Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. Vol.56, No.7 (2021), 769-785. doi:10.1080/10934529.2021.1927421 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77074
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine
Other Contributor(s)
Abstract
The arsenic speciation, the abundance of arsenite-oxidizing bacteria, and microbial community structures in the groundwater, surface water, and soil from a gold mining area were explored using the PHREEQC model, cloning-ddPCR of the aioA gene, and high-throughput sequencing of the 16S rRNA gene, respectively. The analysis of the aioA gene showed that arsenite-oxidizing bacteria retrieved from groundwater, surface water, and soil were associated with Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. In groundwaters from the mining area, there were relatively high ratios of aioA/total 16S rRNA gene copies and the dominance of As5+, which suggested the presence and activity of arsenite-oxidizing bacteria. Metagenomic analysis revealed that the majority of the soil and surface water microbiomes were Proteobacteria, Actinobacteria, Bacteroidetes, and Chloroflexi, whereas the groundwater microbiomes were dominated exclusively by Betaproteobacteria and Alphaproteobacteria. Geochemical factors influencing the microbial structure in the groundwater were As, residence time, and groundwater flowrate, while those showing a positive correlation to the microbial structure in the surface water were TOC, ORP, and DO. This study provides insights into the groundwater, surface water, and soil microbiomes from a gold mine and expands the current understanding of the diversity and abundance of arsenite-oxidizing bacteria, playing a vital role in global As cycling.