Publication: Anti-inflammatory effect of photodynamic therapy using guaiazulene and red lasers on peripheral blood mononuclear cells
Issued Date
2020-09-01
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ISSN
18731597
15721000
15721000
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2-s2.0-85086887537
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Mahidol University
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SCOPUS
Bibliographic Citation
Photodiagnosis and Photodynamic Therapy. Vol.31, (2020)
Suggested Citation
Ampika Phutim-Mangkhalthon, Aroon Teerakapong, Patcharaporn Tippayawat, Noppawan Phumala Morales, Supawich Morkmued, Subin Puasiri, Aroonsri Priprem, Teerasak Damrongrungruang Anti-inflammatory effect of photodynamic therapy using guaiazulene and red lasers on peripheral blood mononuclear cells. Photodiagnosis and Photodynamic Therapy. Vol.31, (2020). doi:10.1016/j.pdpdt.2020.101747 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57681
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Title
Anti-inflammatory effect of photodynamic therapy using guaiazulene and red lasers on peripheral blood mononuclear cells
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Abstract
© 2020 Elsevier B.V. Introduction: Photodynamic therapy improves oral mucositis treatment. The reactive oxygen species (ROS) generated from this reaction could contribute to an anti-inflammatory effect by suppressing inflammatory cells. Objective: To evaluate the anti-inflammatory effect of photodynamic therapy using guaiazulene and a red laser in peripheral blood mononuclear cells (PBMCs). Methods: Guaiazulene solutions (1, 2, 5, 25, 35, and 100 μM in 99.8 % methanol) were irradiated with red laser light (625 nm, 146.2 mW/cm2) in continuous mode at 0, 4, and 8 J/cm2 in black 96-well plates. ROS were measured using spin trapping technique with electron spin resonance (ESR) spectroscopy and fluorescence. The two highest concentrations were tested using cell viability (PrestoBlue®) and anti-inflammation (RANTES and PGE2 ELISA) assay kits. Kruskal-Wallis and Dunn Bonferroni tests were used for statistical analyses with significant differences at p-value < 0.05. Results: Guaiazulene solutions between 2 and 5 μM exposed to red laser light at 4−8 J/cm2 generated significantly more singlet oxygen compared to the no guaiazulene group (p < 0.01) and reduced RANTES and PGE2 levels in TNF-α-inflamed peripheral blood mononuclear cells without affecting cell viability. Conclusion: Photodynamic activation of guaiazulene generated singlet oxygen and suppressed inflammatory markers in PBMCs.