Publication: Influence of immobilized biomolecules on magnetic bead plug formation and retention in capillary electrophoresis
6
Issued Date
2012-03-01
Resource Type
ISSN
15222683
01730835
01730835
Other identifier(s)
2-s2.0-84860241722
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Mahidol University
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SCOPUS
Bibliographic Citation
Electrophoresis. Vol.33, No.5 (2012), 827-833
Suggested Citation
Rachel L. Henken, Rattikan Chantiwas, S. Douglass Gilman Influence of immobilized biomolecules on magnetic bead plug formation and retention in capillary electrophoresis. Electrophoresis. Vol.33, No.5 (2012), 827-833. doi:10.1002/elps.201100353 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13781
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Title
Influence of immobilized biomolecules on magnetic bead plug formation and retention in capillary electrophoresis
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Abstract
Significant changes in the formation and retention of magnetic bead plugs in a capillary during electrophoresis were studied, and it was demonstrated that these effects were due to the type of biological molecule immobilized on the surface of these beads. Three biological molecules, an antibody, an oligonucleotide, and alkaline phosphatase (AP), were attached to otherwise identical streptavidin-coated magnetic beads through biotin-avidin binding in order to isolate differences in bead immobilization in a magnetic field resulting from the type of biological molecule immobilized on the bead surface. AP was also attached to the magnetic beads using epoxy groups on the bead surfaces (instead of avidin-biotin binding) to study the impact of immobilization chemistry. The formation and retention of magnetic bead plugs were studied quantitatively using light scattering detection of magnetic particles eluting from the bead plugs and qualitatively using microscopy. Both the types of biomolecule immobilized on the magnetic bead surface and the chemistry used to link the biomolecule to the magnetic bead impacted the formation and retention of the bead plugs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.