Publication: Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent
Issued Date
2017-04-01
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00399140
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2-s2.0-85009083948
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Mahidol University
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SCOPUS
Bibliographic Citation
Talanta. Vol.165, (2017), 612-618
Suggested Citation
Thapanee Pruksatrakul, Pattamaporn Phoopraintra, Prapin Wilairat, Pimchai Chaiyen, Rattikan Chantiwas Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent. Talanta. Vol.165, (2017), 612-618. doi:10.1016/j.talanta.2017.01.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41901
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Title
Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent
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Abstract
© 2017 Elsevier B.V. Short-chain fatty acids, such as acetic, propionic, butyric, iso-valeric and valeric acids, play an important role in methanogenesis activity for biogas production processes. Thus, simple and rapid procedures for monitoring the levels of short-chain fatty acids are requisite for sustaining biogas production. This work presents the development of a sequential injection-liquid microextraction (SI-LME) procedure with GC-FID analysis for determination of short-chain fatty acids. GC-FID was employed for detection of the short-chain fatty acids. Calibration curves were linear with good coefficients of determination (r2>0.999), using methacrylic acid as the internal standard. Limits of quantification (LOQ) were in the range of 0.03–0.19 mM. The SI-LME procedure employed tert-butyl methyl ether (TBME) as the extracting solvent. Various SI-LME conditions were investigated and optimized to obtain the highest recovery of extraction. With these optimized conditions, an extraction recovery of the five key short-chain fatty acids of 67–90% was obtained, with less than 2% RSD (n=3). The final SI-LME procedure employed two fluidic zones of TBME with a single aqueous fluidic zone of sample sandwiched between the TBME zones, with 5 cycles of flow reversal at a flow rate of 5 µL/s for the extraction process. Intra- and inter-day precision values were 0.5–4.0% RSD and 3.3–4.8% RSD, respectively. Accuracy based on percentage of sample recovery were in the range of 69–96, 102–107, and 82–101% (n=4) for acetic, propionic and butyric acids, respectively. The proposed method was applied for the measurement of short-chain fatty acids in palm oil mill effluents used in biogas production in a factory performing palm oil extraction process. The SI-LME method provides improved extraction performance with high precision, and is both simple and rapid with its economical extraction technique. The SI-LME procedure with GC-FID has strong potential for use as a quality control process for monitoring short-chain fatty acid levels in biogas production.