Publication: Multiplex human Malaria array: Quantifying antigens for malaria rapid diagnostics
Issued Date
2020-06-01
Resource Type
ISSN
14761645
00029637
00029637
Other identifier(s)
2-s2.0-85086051620
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.102, No.6 (2020), 1366-1369
Suggested Citation
Ihn Kyung Jang, Abby Tyler, Chris Lyman, John C. Rek, Emmanuel Arinaitwe, Harriet Adrama, Maxwell Murphy, Mallika Imwong, Stephane Proux, Warat Haohankhunnatham, Rebeccar Barney, Andrew Rashid, Michael Kalnoky, Maria Kahn, Allison Golden, François Nosten, François Nosten, Bryan Greenhouse, Dionicia Gamboa, Gonzalo J. Domingo Multiplex human Malaria array: Quantifying antigens for malaria rapid diagnostics. American Journal of Tropical Medicine and Hygiene. Vol.102, No.6 (2020), 1366-1369. doi:10.4269/ajtmh.19-0763 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57975
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Title
Multiplex human Malaria array: Quantifying antigens for malaria rapid diagnostics
Author(s)
Ihn Kyung Jang
Abby Tyler
Chris Lyman
John C. Rek
Emmanuel Arinaitwe
Harriet Adrama
Maxwell Murphy
Mallika Imwong
Stephane Proux
Warat Haohankhunnatham
Rebeccar Barney
Andrew Rashid
Michael Kalnoky
Maria Kahn
Allison Golden
François Nosten
François Nosten
Bryan Greenhouse
Dionicia Gamboa
Gonzalo J. Domingo
Abby Tyler
Chris Lyman
John C. Rek
Emmanuel Arinaitwe
Harriet Adrama
Maxwell Murphy
Mallika Imwong
Stephane Proux
Warat Haohankhunnatham
Rebeccar Barney
Andrew Rashid
Michael Kalnoky
Maria Kahn
Allison Golden
François Nosten
François Nosten
Bryan Greenhouse
Dionicia Gamboa
Gonzalo J. Domingo
Other Contributor(s)
Abstract
© 2020 by The American Society of Tropical Medicine and Hygiene. Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generationRDTdevelopment and screen asymptomatic malaria,we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparumspecific lactate dehydrogenase (Pf LDH), Plasmodium vivax-specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum.