Publication: Antiviral action of nitric oxide on dengue virus type 2 replication
Issued Date
2006-10-01
Resource Type
ISSN
00221317
Other identifier(s)
2-s2.0-33749066767
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of General Virology. Vol.87, No.10 (2006), 3003-3011
Suggested Citation
Ratree Takhampunya, R. Padmanabhan, Sukathida Ubol Antiviral action of nitric oxide on dengue virus type 2 replication. Journal of General Virology. Vol.87, No.10 (2006), 3003-3011. doi:10.1099/vir.0.81880-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23301
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antiviral action of nitric oxide on dengue virus type 2 replication
Author(s)
Other Contributor(s)
Abstract
Recently, nitric oxide (NO) has been shown to suppress dengue virus (DENV) RNA and protein accumulaton in infected cells. In this report, the potential target of the inhibitory effect of NO was studied at the molecular level. The NO donor, S-nitroso-N-acetylpenicillamine (SNAP), showed an inhibitory effect on RNA accumulation at around 8-14 h post-infection, which corresponded to the step of viral RNA synthesis in the DENV life cycle. The activity of the viral replicase isolated from SNAP-treated DENV-2-infected cells was suppressed significantly compared with that of the negative-control N-acetyl-DL-penicillamine (NAP)-treated cells. Further investigations on the molecular target of NO action showed that the activity of recombinant DENV-2 NS5 in negative-strand RNA synthesis was affected in the presence of 5 mM SNAP in in vitro RNA-dependent RNA polymerase (RdRp) assays, whereas the RNA helicase activity of DENV-2 NS3 was not inhibited up to a concentration of 15 mM SNAP. These results suggest that the inhibitory effect of NO on DENV infection is partly via inhibition of the RdRp activity, which then downregulates viral RNA synthesis. © 2006 SGM.