Publication: Purification and characterization of three β-glycosidases exhibiting high glucose tolerance from Aspergillus niger ASKU28
Issued Date
2014-01-01
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ISSN
13476947
09168451
09168451
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2-s2.0-84930706404
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.78, No.7 (2014), 1167-1176
Suggested Citation
Preeyanuch Thongpoo, Chantragan Srisomsap, Daranee Chokchaichamnankit, Vichien Kitpreechavanich, Jisnuson Svasti, Prachumporn T. Kongsaeree Purification and characterization of three β-glycosidases exhibiting high glucose tolerance from Aspergillus niger ASKU28. Bioscience, Biotechnology and Biochemistry. Vol.78, No.7 (2014), 1167-1176. doi:10.1080/09168451.2014.915727 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33466
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Title
Purification and characterization of three β-glycosidases exhibiting high glucose tolerance from Aspergillus niger ASKU28
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Abstract
© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry. Production and utilization of cellulosic ethanol has been limited, partly due to the difficulty in degradation of cellulosic feedstock. β-Glucosidases convert cellobiose to glucose in the final step of cellulose degradation, but they are inhibited by high concentrations of glucose. Thus, in this study, we have screened, isolated, and characterized three β-glycosidases exhibiting highly glucose-tolerant property from Aspergillus niger ASKU28, namely β-xylosidase (P1.1), β-glucosidase (P1.2), and glucan 1,3-β-glucosidase (P2). Results from kinetic analysis, inhibition study, and hydrolysis of oligosaccharide substrates supported the identification of these enzymes by both LC/MS/MS analysis and nucleotide sequences. Moreover, the highly efficient P1.2 performed better than the commercial β-glucosidase preparation in cellulose saccharification, suggesting its potential applications in the cellulosic ethanol industry. These results shed light on the nature of highly glucose-tolerant β-glucosidase activities in A. niger, whose kinetic properties and identities have not been completely determined in any prior investigations.