Publication:
Analysis of sequence variation in Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis and DNA sequence

Issued Date

2005-01-01

Resource Type

Article

ISSN

13835769

DOI

10.1016/j.parint.2004.12.002

Other identifier(s)

2-s2.0-13844257165

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Parasitology International. Vol.54, No.1 (2005), 65-68

Suggested Citation

Charinthon Ngarmamonpirat, Jitra Waikagul, Songsak Petmitr, Paron Dekumyoy, Wichit Rojekittikhun, Malinee T. Anantapruti Analysis of sequence variation in Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis and DNA sequence. Parasitology International. Vol.54, No.1 (2005), 65-68. doi:10.1016/j.parint.2004.12.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16614

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Title

Analysis of sequence variation in Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis and DNA sequence

Author(s)

Charinthon Ngarmamonpirat
 Jitra Waikagul
 Songsak Petmitr
 Paron Dekumyoy
 Wichit Rojekittikhun
 Malinee T. Anantapruti

Other Contributor(s)

Mahidol University

Abstract

Morphological variations were observed in the advance third stage larvae of Gnathostoma spinigerum collected from swamp eel (Fluta alba), the second intermediate host. Larvae with typical and three atypical types were chosen for partial cytochrome c oxidase subunit I (COI) gene sequence analysis. A 450 bp polymerase chain reaction product of the COI gene was amplified from mitochondrial DNA. The variations were analyzed by single-strand conformation polymorphism and DNA sequencing. The nucleotide variations of the COI gene in the four types of larvae indicated the presence of an intra-specific variation of mitochondrial DNA in the G. spinigerum population. © 2005 Elsevier Ireland Ltd. All rights reserved.

Keyword(s)

Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/16614

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Scopus 2001-2005