Publication:
Using Lopinavir Concentrations in Hair Samples to Assess Treatment Outcomes on Second-Line Regimens among Asian Children

Issued Date

2015-01-01

Resource Type

Article

ISSN

19318405
08892229

DOI

10.1089/aid.2015.0111

Other identifier(s)

2-s2.0-84941950858

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Mahidol University

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SCOPUS

Bibliographic Citation

AIDS Research and Human Retroviruses. Vol.31, No.10 (2015), 1009-1014

Suggested Citation

Wasana Prasitsuebsai, Stephen J. Kerr, Khanh Huu Truong, Jintanat Ananworanich, Viet Chau Do, Lam Van Nguyen, Nia Kurniati, Pope Kosalaraksa, Tavitiya Sudjaritruk, Kulkanya Chokephaibulkit, Narukjaporn Thammajaruk, Thida Singtoroj, Sirinya Teeraananchai, Howard Horng, Peter Bacchetti, Monica Gandhi, Annette H. Sohn Using Lopinavir Concentrations in Hair Samples to Assess Treatment Outcomes on Second-Line Regimens among Asian Children. AIDS Research and Human Retroviruses. Vol.31, No.10 (2015), 1009-1014. doi:10.1089/aid.2015.0111 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36182

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Title

Using Lopinavir Concentrations in Hair Samples to Assess Treatment Outcomes on Second-Line Regimens among Asian Children

Author(s)

Wasana Prasitsuebsai
 Stephen J. Kerr
 Khanh Huu Truong
 Jintanat Ananworanich
 Viet Chau Do
 Lam Van Nguyen
 Nia Kurniati
 Pope Kosalaraksa
 Tavitiya Sudjaritruk
 Kulkanya Chokephaibulkit
 Narukjaporn Thammajaruk
 Thida Singtoroj
 Sirinya Teeraananchai
 Howard Horng
 Peter Bacchetti
 Monica Gandhi
 Annette H. Sohn

Other Contributor(s)

The HIV Netherlands Australia Thailand Research Collaboration
 Children's Hospital 1
 Walter Reed Army Institute of Research
 HJF
 Children's Hospital 2
 National Hospital of Pediatrics Hanoi
 University of Indonesia, RSUPN Dr. Cipto Mangunkusumo
 Khon Kaen University
 Chiang Mai University
 Mahidol University
 TREAT Asia/amfAR-The Foundation for AIDS Research
 University of California, San Francisco

Abstract

© 2015, Mary Ann Liebert, Inc. We conducted a prospective monitoring study to determine whether antiretroviral (ARV) levels in hair of Asian children on second-line protease inhibitor-based ARV therapy (ART) are associated with virologic failure (VF), compared to plasma drug levels and self-reported adherence. HIV-infected Asian children on second-line ART regimens were enrolled into a longitudinal cohort. Traditional adherence measures, plasma, and hair samples were collected 24 weeks after study enrollment. Hair ARV levels were determined via liquid chromatography/tandem mass spectrometry. Among 149 children on lopinavir/ritonavir-based regimens, 47% were female; the median [interquartile range (IQR)] age was 10.3 (7.9-13.3) years. The median CD4% was 26% (IQR 21.7-32.1%) and the median CD4 cell count 754 (IQR 596-1,013) cells/mm<sup>3</sup>. The median duration of lopinavir-based ART prior to week 24 of the study was 2.9 (IQR 1.6-4.2) years. Adherence was >95% in 91% (135/148) by visual analogue scale and 89% (129/145) by pill count. The median lopinavir hair concentrations were 5.43 (IQR 3.21-9.01) ng/mg in children with HIV RNA >1,000 copies/ml and 9.96 (IQR 6.51-12.31) ng/mg in children with HIV RNA <1,000 copies/ml (p = 0.003). Plasma trough and lopinavir hair concentrations were not statistically significantly correlated (Pearson's correlation coefficient 0.20; p = 0.13). Increasing lopinavir hair concentrations in quartiles were strongly associated with virologic success (odds ratios ≥4.0, overall p = 0.02), while self-reported adherence, pill count, and plasma lopinavir levels were not. Based on this first report of hair ARV concentrations and virologic outcomes in children, ARV hair concentrations, representing longer-term adherence, may be useful to identify children at risk for VF.

Keyword(s)

Immunology and Microbiology

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URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/36182

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Scopus 2011-2015