Publication:
Gossypol prevents activation of purified proenzyme of human seminal plasma acidic proteinase

Issued Date

1986-09-04

Resource Type

Article

ISSN

03044165

DOI

10.1016/0304-4165(86)90318-1

Other identifier(s)

2-s2.0-0022554058

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

BBA - General Subjects. Vol.883, No.2 (1986), 271-276

Suggested Citation

Likasit Vongsorasak, Jisnuson Svasti Gossypol prevents activation of purified proenzyme of human seminal plasma acidic proteinase. BBA - General Subjects. Vol.883, No.2 (1986), 271-276. doi:10.1016/0304-4165(86)90318-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9666

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Title

Gossypol prevents activation of purified proenzyme of human seminal plasma acidic proteinase

Author(s)

Likasit Vongsorasak
 Jisnuson Svasti

Other Contributor(s)

Mahidol University

Abstract

Gossypol inhibits the potential activity of the proenzyme form of human seminal plasma acidic proteinase, but has no effect on the active enzyme under the conditions tested. Inhibition of proenzyme is rapid and pH-dependent: 50% inhibition can be observed at gossypol concentrations of approx. 1.5 · 10 -5 M. SDS-polyacrylamide gel electrophoresis indicates that treatment of proenzyme with gossypol prevents the formation of active enzyme that normally occurs on acidification. Determination of free amino groups with 1-fluoro-2,4-dinitrobenzene suggests that gossypol reacts with 7.8 out of the 11.0 lysine residues in proenzyme: such a reaction could interfere with the activation process. © 1986.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/9666

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Scopus 1969-1990