Publication: Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16
Issued Date
2019-12-01
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17454514
01458884
01458884
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2-s2.0-85074275833
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Food Biochemistry. Vol.43, No.12 (2019)
Suggested Citation
Thitinan Kitisin, Worawit Suphamungmee, Krai Meemon Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. Journal of Food Biochemistry. Vol.43, No.12 (2019). doi:10.1111/jfbc.13075 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/49693
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Title
Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16
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Abstract
© 2019 Wiley Periodicals, Inc. Saponins are secondary metabolite compounds that can be found in sea cucumbers (Holothuroidea spp.). However, little is known about how saponin-rich extracts from Holothuria leucospilota can delay and prolong the lifespan of the whole organism. In this study, anti-aging effects of H. leucospilota extracts were studied on Caenorhabditis elegans. NMR analysis revealed that body wall n-butanol-extract of H. leucospilota (BW-BU) is saponin-rich. BW-BU extracts exhibited antioxidant activities by 2,2ʹ-diphenyl-2-picrylhydrazyl assay (EC50 = 10.23 ± 0.12 mg/ml) and 2,2ʹ-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid assay (EC50 = 3.91 ± 0.04 mg/ml). BW-BU extracts increased lifespan of L4 and L1 C. elegans (5.92% and 15.76%, respectively), which also increased worm growth, stress resistance, and reduced biomarkers for aging. BW-BU extracts activated DAF-16 nuclear localization and upregulated daf-16 and DAF-16 target genes expression. Taken together, this study revealed the evidences on anti-aging activities of saponin-rich extracts from H. leucospilota, which can extend lifespan of C. elegans via daf-16. Practical applications: In recent years, age-associated chronic diseases have had a significant impact on quality of life. Many natural compounds exhibit anti-aging activities, especially in sea cucumber, H. leucospilota. Our results indicated that H. leucospilota is good for health. Extracts from H. leucospilota contain a bioactive compound that can be potentially used to promote longevity and disease prevention in aging.