Publication: Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae
| dc.contributor.author | Boonhiang Promdonkoy | en_US |
| dc.contributor.author | Patcharee Promdonkoy | en_US |
| dc.contributor.author | Sakol Panyim | en_US |
| dc.contributor.other | Thailand National Center for Genetic Engineering and Biotechnology | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-06-21T08:07:46Z | |
| dc.date.available | 2018-06-21T08:07:46Z | |
| dc.date.issued | 2005-11-01 | en_US |
| dc.description.abstract | Cry4Ba is a δ-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic δ-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC50= 140 ng ml-1) but virtually inactive to Culex quinquefasciatus larvae. Cyt2Aa2 expressed in E. coli exhibited moderate activity against A. aegypti and C. quinquefasciatus larvae with LC50values of 350 and 250 ng ml-1, respectively. Co-expression of both toxins in E. coli dramatically increased toxicity to both A. aegypti andC. quinquefasciatus larvae (LC50= 7 and 20 ng ml-1, respectively). This is the first report to demonstrate that Cry4Ba and Cyt2Aa2 have high synergistic activity against C. quinquefasciatus larvae. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. | en_US |
| dc.identifier.citation | FEMS Microbiology Letters. Vol.252, No.1 (2005), 121-126 | en_US |
| dc.identifier.doi | 10.1016/j.femsle.2005.08.038 | en_US |
| dc.identifier.issn | 03781097 | en_US |
| dc.identifier.other | 2-s2.0-26844503739 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/16279 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=26844503739&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.subject | Immunology and Microbiology | en_US |
| dc.title | Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=26844503739&origin=inward | en_US |