Publication: Reverse passive hemagglutination tests for rapid diagnosis of snake envenomation
7
Issued Date
1993-09-01
Resource Type
ISSN
01971522
Other identifier(s)
2-s2.0-0027318229
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Immunoassay. Vol.14, No.3 (1993), 105-127
Suggested Citation
Leera Kittigul, Kavi Ratanabanangkoon Reverse passive hemagglutination tests for rapid diagnosis of snake envenomation. Journal of Immunoassay. Vol.14, No.3 (1993), 105-127. doi:10.1080/15321819308019844 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22580
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Reverse passive hemagglutination tests for rapid diagnosis of snake envenomation
Author(s)
Other Contributor(s)
Abstract
Reverse passive hemagglutination (RPHA) tests for the detection of six major poisonous snake venoms of Thailand were studied. Three different species of red blood cells i.e., sheep (SRBC), human (HRBC) and chicken (CRBC) were sensitized with protein A-affinity purified rabbit antivenom IgG using chromic chloride as a coupling reagent. The properties of these sensitized erythrocytes with regard to sensitivity, specificity, stability to venom enzymes and storage etc., were studied and compared. The sensitivities of the RPHA tests in venom detection were 2 to 635 ng/ml. Cross-reactions were observed with heterologous venoms at concentrations at least 62 times higher than those observed with homologous venoms. After treatment with glutaraldehyde, the coupled red blood cells showed reduced. sensitivity but were stable at 4 C from 1 to 12 months depending upon the antibody and the species of erythrocytes. The entire test required 60 to 120 min. The RPHA using fresh SRBC correctly identified various venoms in 48 of 59 (81.3%) serum samples and 16 of 26 (61.5%) wound swabs. Venom mis-identifications were made in 2 sera (3.4%). In a comparison of 24 paired serum and wound swab samples, more positive identifications were made with serum than with swab samples but the difference was not statistically significant (p > 0.05). © 1993, Taylor & Francis Group, LLC. All rights reserved.