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Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species

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dc.contributor.authorPaweena Thuwanuten_US
dc.contributor.authorSayamon Srisuwatanasagulen_US
dc.contributor.authorGrisnarong Wongbandueen_US
dc.contributor.authorNae Tanpraditen_US
dc.contributor.authorAmpika Thongpakdeeen_US
dc.contributor.authorDaraka Tongthainanen_US
dc.contributor.authorSukanya Manee-inen_US
dc.contributor.authorKaywalee Chatdarongen_US
dc.contributor.otherChulalongkorn Universityen_US
dc.contributor.otherZoological Park Organization, Bangkoken_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-10-19T04:29:21Z
dc.date.available2018-10-19T04:29:21Z
dc.date.issued2013-10-01en_US
dc.description.abstractThis study compared the effects of slow and fast freezing of testicular tissue of wild animals collected at post-mortem on testicular structure and testicular sperm. The testes of seven animals that had died in captivity; three felids (jungle cat, lion and leopard), two cervids (rusa deer and fea's muntjac) and two bovids (Sumatran serows) were cryopreserved using slow- and fast-freezing protocols. There were greater reductions in the integrity of the sperm membrane and DNA in tissues cryopreserved using slow freezing compared to fast freezing (membrane integrity reduced by 21.5. ±. 12.4% vs. 13.0. ±. 6.9%, P= 0.11 and DNA integrity reduced by 22.7. ±. 16.3% vs. 6.6. ±. 6.3%, P= 0.13). Histologically, there were similar degrees of detachment and shrinkage of the seminiferous tubules whereas, TUNEL assay revealed a tendency towards more apoptotic changes in the intra-tubular cells of tissues frozen using fast freezing compared to slow freezing (P= 0.09). In conclusion, fast freezing tended to cause less damage to testicular sperm but its protective effect on intra-tubular cells was likely compromised. This is the first report of gamete recovery in the wild and of the comparison in various wildlife species, between testicular tissues cryopreserved using different protocols. © 2013 Elsevier Inc.en_US
dc.identifier.citationCryobiology. Vol.67, No.2 (2013), 244-247en_US
dc.identifier.doi10.1016/j.cryobiol.2013.07.002en_US
dc.identifier.issn10902392en_US
dc.identifier.issn00112240en_US
dc.identifier.other2-s2.0-84883555550en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14594/30978
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883555550&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectMedicineen_US
dc.titleSperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild speciesen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883555550&origin=inwarden_US
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