Publication: The effect of botulinum toxin type A in different dilution on the contraction of fibroblast—In vitro study
Issued Date
2019-10-01
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video/youtube
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14732165
14732130
14732130
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2-s2.0-85069879480
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Cosmetic Dermatology. Vol.18, No.5 (2019), 1215-1223
Suggested Citation
Rungsima Wanitphakdeedecha, Arisa Kaewkes, Chanida Ungaksornpairote, Saowalak Limsaengurai, Uraiwan Panich, Woraphong Manuskiatti The effect of botulinum toxin type A in different dilution on the contraction of fibroblast—In vitro study. Journal of Cosmetic Dermatology. Vol.18, No.5 (2019), 1215-1223. doi:10.1111/jocd.13058 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51380
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Title
The effect of botulinum toxin type A in different dilution on the contraction of fibroblast—In vitro study
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Abstract
© 2019 The Authors. Journal of Cosmetic Dermatology Published by Wiley Periodicals, Inc. Background: Botulinum toxin type A (BoNT-A) may directly remodel dermal tissues or induce a loss of normal morphology and cytoplasmic retraction and spread. Intradermal injection was claimed to produce a dermo-lifting effect, including midface lifting by using low concentration with variable dilution. Objective: To understand how intradermal BoNT-A achieves tissue lifting, we examined different type of BoNT-A and their effects on dermal fibroblast contraction. Methods: Normal human dermal fibroblasts were treated with onabotulinumtoxin (ONA), abobotulinumtoxin (ABO), prabotulinumtoxinA (PRABO), incobotulinumtoxinA (INCO), and letibotulinumtoxin A (LETI) in dilutions used in real-world practice. Fifty fibroblasts per dilution were photographed and measured the length to demonstrate their contraction every 2 hours from baseline (0 hours) to 12 hours post-treatment. Results: ONA did not significantly decrease fibroblast lengths, at any timepoint or dilution. At 1:7 dilution ratios, ABO decreased fibroblast lengths after 2 hours and significantly after 10-12 hours. At 1:7, 1:8, 1:9, and 1:10 dilution, PRABO decreased length, and most rapidly at 1:7 and 1:8. At 1:6, 1:8, 1:9, and 1:10 dilution, INCO decreased lengths almost immediately. At 1:6 dilution, INCO decreased lengths almost immediately. At 1:7 dilution, INCO decreased lengths after 2-4 hours, while at 1:8, 1:9, and 1:10 dilution, INCO decreased lenghts nearly imediately. LETI decreased lengths at all dilutions except 1:9, with near-immediate effects at 1:6, 1:7, 1:8, and 1:10. At 1:4 dilution, LETI decreased lengths from 1 hour. Conclusions: Different commercial preparations of BoNT-A toxins cause different fibroblast contractions in vitro. Product selection and dilution used may affect the clinical outcome of intradermal injection of BoNT-A for face lifting.