Publication: Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle
Issued Date
2010-12-01
Resource Type
ISSN
14220067
Other identifier(s)
2-s2.0-78650985401
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences. Vol.11, No.12 (2010), 5323-5338
Suggested Citation
Suwit Duangmano, Sumana Dakeng, Weena Jiratchariyakul, Apichart Suksamrarn, Duncan R. Smith, Pimpicha Patmasiriwat Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle. International Journal of Molecular Sciences. Vol.11, No.12 (2010), 5323-5338. doi:10.3390/ijms11125323 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28579
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle
Other Contributor(s)
Abstract
Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (T47D, SKBR-3, and MCF-7) and a mammary epithelium cell line (HBL-100). Cell viability after treatment with cucurbitacin B, which is an active ingredient of this herb, was assessed. Telomeric Repeat Amplification Protocol (TRAP) assays and RT-PCR (qualitative and realtime) were performed to investigate activity of telomerase as well as expression of human telomerase reverse transcriptase (hTERT) and c-Myc. The c-Myc protein level was also determined in SKBR-3 and HBL-100 cells. Our results show that the cucurbitacin B inhibits growth and telomerase activity in the three breast cancer cell lines and exerts an obvious inhibitory effect in the estrogen receptor (ER)-negative breast cancer SKBR-3 cells. The expression of hTERT and c-Myc were also inhibited by cucurbitacin B, In addition, a clear reduction of c-Myc protein was observed after treatment in SKBR-3 cells even with a concentration of cucurbitacin B that was ten-times lower compared to the concentration used for HBL-100. Our findings imply that cucurbitacin B exerts an anticancer effect by inhibiting telomerase via down regulating both the hTERT and c-Myc expression in breast cancer cells. © 2010 by the authors.