Publication: Impaired trafficking and intracellular retention of mutant kidney anion exchanger 1 proteins (G701D and A858D) associated with distal renal tubular acidosis
Issued Date
2010-04-17
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ISSN
14645203
09687688
09687688
Other identifier(s)
2-s2.0-77950202878
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular Membrane Biology. Vol.27, No.2-3 (2010), 92-103
Suggested Citation
Duangporn Ungsupravate, Nunghathai Sawasdee, Sookkasem Khositseth, Wandee Udomchaiprasertkul, Siri Khoprasert, Jing Li, Reinhart A.F. Reithmeier, Pa Thai Yenchitsomanus Impaired trafficking and intracellular retention of mutant kidney anion exchanger 1 proteins (G701D and A858D) associated with distal renal tubular acidosis. Molecular Membrane Biology. Vol.27, No.2-3 (2010), 92-103. doi:10.3109/09687681003588020 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28735
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Title
Impaired trafficking and intracellular retention of mutant kidney anion exchanger 1 proteins (G701D and A858D) associated with distal renal tubular acidosis
Abstract
Novel compound heterozygous mutations, G701D, a recessive mutation, and A858D, a mild dominant mutation, of human solute carrier family 4, anion exchanger, member 1 (SLC4A1) were identified in two pediatric patients with distal renal tubular acidosis (dRTA). To examine the interaction, trafficking, and cellular localization of the wild-type and two mutant kidney AE1 (kAE1) proteins, we expressed the proteins alone or together in human embryonic kidney (HEK) 293T and Madin-Darby canine kidney (MDCK) epithelial cells. In individual expressions, wild-type kAE1 was localized at the cell surface of HEK 293T and the basolateral membrane of MDCK cells. In contrast, kAE1 G701D was mainly retained intracellularly, while kAE1 A858D was observed intracellularly and at the cell surface. In co-expression experiments, wild-type kAE1 formed heterodimers with kAE1 G701D and kAE1 A858D, and promoted the cell surface expression of the mutant proteins. The co-expressed kAE1 G701D and A858D could also form heterodimers but showed predominant intracellular retention in HEK 293T and MDCK cells. Thus impaired trafficking of the kAE1 G701D and A858D mutants would lead to a profound decrease in functional kAE1 at the basolateral membrane of α-intercalated cells in the distal nephron of the patients with dRTA. © 2010 Informa UK Ltd.