Publication:
Propagation of infectious yellow head virus particles prior to cytopathic effect in primary lymphoid cell cultures of Penaeus monodon

5

Issued Date

2003-08-04

Resource Type

Article

ISSN

01775103

DOI

10.3354/dao055253

Other identifier(s)

2-s2.0-0042194959

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Diseases of Aquatic Organisms. Vol.55, No.3 (2003), 253-258

Suggested Citation

Wanchai Assavalapsakul, Duncan R. Smith, Sakol Panyim Propagation of infectious yellow head virus particles prior to cytopathic effect in primary lymphoid cell cultures of Penaeus monodon. Diseases of Aquatic Organisms. Vol.55, No.3 (2003), 253-258. doi:10.3354/dao055253 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20625

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Title

Propagation of infectious yellow head virus particles prior to cytopathic effect in primary lymphoid cell cultures of Penaeus monodon

Author(s)

Wanchai Assavalapsakul
 Duncan R. Smith
 Sakol Panyim

Other Contributor(s)

Mahidol University

Abstract

Yellow head virus is a highly pathogenic agent that can cause a fatal disease in several species of penaeid shrimps. Using a primary cell culture and an in vitro quantal assay (TCID50), this study sought to determine the propagation profile of yellow head virus after inoculation at a low multiplicity of infection in the lymphoid tissue (oka organ) of Penaeus monodon. Detectable levels of virus were present as early as 24 h post-inoculation. Maximal viral yields were reached by 4 d post-infection, approximately 24 h after the onset of a detectable cytopathic effect, which was normally observable at 3 d post-inoculation. The methodology provides a useful tool for studying yellow head virus-host-cell interactions.

Keyword(s)

Agricultural and Biological Sciences

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URI

https://repository.li.mahidol.ac.th/handle/123456789/20625

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Scopus 2001-2005