Publication:
Comparison of restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from cerebrospinal fluid and sputum: a preliminary report

Issued Date

1993-01-01

Resource Type

Article

ISSN

09628479

DOI

10.1016/0962-8479(93)90013-N

Other identifier(s)

2-s2.0-0027314642

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Tubercle and Lung Disease. Vol.74, No.3 (1993), 204-207

Suggested Citation

P. Palittapongarnpim, S. Rienthong, W. Panbangred Comparison of restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from cerebrospinal fluid and sputum: a preliminary report. Tubercle and Lung Disease. Vol.74, No.3 (1993), 204-207. doi:10.1016/0962-8479(93)90013-N Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22590

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Title

Comparison of restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from cerebrospinal fluid and sputum: a preliminary report

Author(s)

P. Palittapongarnpim
 S. Rienthong
 W. Panbangred

Other Contributor(s)

Mahidol University
 Thailand Ministry of Public Health

Abstract

Strain characterization of Mycobacterium tuberculosis has been based mainly on mycobacteriophage typing or chromosomal DNA restriction fragment analysis. In this study 10 randomly selected EcoRI chromosomal DNA fragments of M. tuberculosis H37RV were labelled with digoxigenin and used to probe the Southern blot preparation of EcoRI or BstEII digested chromosomal DNA of clinical isolates of M. tuberculosis. 2 probes were able to reveal restriction fragment length polymorphism. Each of the probes divided 15 pulmonary and 6 cerebrospinal fluid (CSF) isolates of M. tuberculosis into 3 groups and combination of both probes divided them into 4 groups. All of the 6 CSF isolates belonged to 1 group while only 5 of the 15 pulmonary isolates belonged to the same group. Work is continuing in order to characterize the nature of the probe and confirm the results in a larger population. © 1993.

Keyword(s)

Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/22590

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Scopus 1991-2000