Publication: Optimization of DNA recovery from toothbrushes
Issued Date
2008-08-01
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ISSN
18751768
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2-s2.0-50349085219
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Mahidol University
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SCOPUS
Bibliographic Citation
Forensic Science International: Genetics Supplement Series. Vol.1, No.1 (2008), 9-10
Suggested Citation
Achirapa Bandhaya, Nathinee Panvisavas Optimization of DNA recovery from toothbrushes. Forensic Science International: Genetics Supplement Series. Vol.1, No.1 (2008), 9-10. doi:10.1016/j.fsigss.2007.10.053 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18877
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Title
Optimization of DNA recovery from toothbrushes
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Abstract
In human identification, the victim's toothbrush is an invaluable personal item as the deposited cellular material contains DNA from which a reference profile can be produced. The profile obtained then allows direct comparison to be made with the profile from the unidentified body. This study was undertaken to determine the minimum number of bristle bundles that would generate a complete DNA profile. The minimum period of usage for a toothbrush to retain enough cells for genotyping was also investigated. We also tested two commonly used DNA extraction methods: QIAamp®DNA Mini Kit and Chelex®100 to explore the efficiency of these protocols in recovering DNA from toothbrushes. In this experiment, volunteers brushed their teeth for 1, 7, 14, or 30 days. DNA was extracted from 5 and 10 bundles of bristles cut from the collected toothbrushes. The amount of DNA recovered was quantified by quantitative real-time PCR, and DNA genotyping was performed for each sample. Data revealed that QIAamp®DNA Mini Kit performed better at yielding DNA in terms of purity, quantity, and quality than Chelex®100. It was also found that, with a suitable method of recovery, DNA samples from five bundles of bristles from all of the toothbrushes generated complete profiles. Based on the experimental results, a general guideline concerning the appropriate extraction method and the quantity of the starting material for the analysis of DNA from toothbrushes could be suggested. © 2008 Elsevier Ireland Ltd. All rights reserved.