Publication: Surface proteomics and label-free quantification of leptospira interrogans serovar pomona
Issued Date
2021-11-01
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ISSN
19352735
19352727
19352727
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2-s2.0-85120676717
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS Neglected Tropical Diseases. Vol.15, No.11 (2021)
Suggested Citation
Teerasit Techawiwattanaboon, Praparat Thaibankluay, Chahya Kreangkaiwal, Suwitra Sathean-Anan-kun, Prasong Khaenam, Jiradej Makjaroen, Trairak Pisitkun, Kanitha Patarakul Surface proteomics and label-free quantification of leptospira interrogans serovar pomona. PLoS Neglected Tropical Diseases. Vol.15, No.11 (2021). doi:10.1371/journal.pntd.0009983 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77691
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Title
Surface proteomics and label-free quantification of leptospira interrogans serovar pomona
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Abstract
Leptospirosis is a re-emerging zoonosis with a global distribution. Surface-exposed outer membrane proteins (SE-OMPs) are crucial for bacterial–host interactions. SE-OMPs locate and expose their epitope on cell surface where is easily accessed by host molecules. This study aimed to screen for surface-exposed proteins and their abundance profile of pathogenic Leptospira interrogans serovar Pomona. Two complementary approaches, surface biotinylation and surface proteolytic shaving, followed by liquid chromatography tandemmass spectrometry (LC-MS/MS) were employed to identify SE-OMPs of intact leptospires. For quantitative comparison, in-depth label-free analysis of SE-OMPs obtained from each method was performed using MaxQuant. The total number of proteins identified was 1,001 and 238 for surface biotinylation and proteinase K shaving, respectively. Among these, 39 were previously known SE-OMPs and 68 were predicted to be localized on the leptospiral surface. Based on MaxQuant analysis for relative quantification, six known SE-OMPs including EF-Tu, LipL21, LipL41, LipL46, Loa22, and OmpL36, and one predicted SEOMPs, LipL71 were found in the 20 most abundant proteins, in which LipL41 was the highest abundant SE-OMP. Moreover, uncharacterized LIC14011 protein (LIP3228 ortholog in serovar Pomona) was identified as a novel predicted surface βb-OMP. High-abundance leptospiral SE-OMPs identified in this study may play roles in virulence and infection and are potential targets for development of vaccine or diagnostic tests for leptospirosis.