Publication: Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum
2
Issued Date
2012-01-15
Resource Type
ISSN
18734235
09565663
09565663
Other identifier(s)
2-s2.0-84455208100
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biosensors and Bioelectronics. Vol.31, No.1 (2012), 562-566
Suggested Citation
Pattarachaya Preechakasedkit, Kulwadee Pinwattana, Wijitar Dungchai, Weena Siangproh, Wanpen Chaicumpa, Pongsri Tongtawe, Orawon Chailapakul Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum. Biosensors and Bioelectronics. Vol.31, No.1 (2012), 562-566. doi:10.1016/j.bios.2011.10.031 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13825
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum
Abstract
An immunochromatographic strip test using gold nanoparticles was developed for the rapid detection of Salmonella typhi (S. typhi) in human serum. The strip test based on the principle of sandwich immunoassay by the specific binding of antigens from S. typhi O901 and antibody of S. typhi O901 on a nitrocellulose membrane. Antibody-gold nanoparticle conjugate was used as the label and was coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and control zone, antibody of S. typhi O901 and an anti-IgG were dotted on the nitrocellulose membrane, respectively. Positive samples were displayed as red dots at the test and control zones of the nitrocellulose membrane, while negative samples resulted in a red dot only in the control zone. The limit of detection (LOD) was found to be 1.14×10 5 cfumL -1 , which could be visually detected by the naked eye within 15min. This strip test provided a lower detection limit and analysis time than a dot blot immunoassay (8.88×10 6 cfumL -1 for LOD and 110min for reaction time). In addition, our immunochromatographic strip test was employed to detect S. typhi in human serum effectively, with high accuracy. This strip test offers great promise for a rapid, simple and low-cost analysis of S. typhi. © 2011 Elsevier B.V.