Publication: A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
Issued Date
2017-01-27
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ISSN
11782013
11769114
11769114
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2-s2.0-85011620491
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Nanomedicine. Vol.12, (2017), 885-898
Suggested Citation
Boonnada Pamornpathomkul, Adisak Wongkajornsilp, Wanida Laiwattanapaisal, Theerasak Rojanarata, Praneet Opanasopit, Tanasait Ngawhirunpat A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin. International Journal of Nanomedicine. Vol.12, (2017), 885-898. doi:10.2147/IJN.S125945 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42047
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Title
A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
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Abstract
© 2017 Pamornpathomkul et al. The aim of this study was to investigate the use of different types of microneedles (MNs) and nanocarriers for in vitro skin permeation and in vivo immunization of plasmid DNA encoding ovalbumin (pOVA). In vitro skin permeation studies indicated that hollow MNs had a superior enhancing effect on skin permeation compared with solid MN patches, electroporation (EP) patches, the combination of MN and EP patches, and untreated skin. Upon using hollow MNs combined with nanocarriers for pOVA delivery, the skin permeation was higher than for the delivery of naked pOVA, as evidenced by the increased amount of pOVA in Franz diffusion cells and immunoglobulin G (IgG) antibody responses. When the hollow MNs were used for the delivery of nanocarrier:pOVA complexes into the skin of mice, they induced a stronger IgG immune response than conventional subcutaneous (SC) injections. In addition, immunization of mice with the hollow MNs did not induce signs of skin infection or pinpoint bleeding. Accordingly, the hollow MNs combined with a nanocarrier delivery system is a promising approach for delivering pOVA complexes to the skin for promoting successful immunization.