Publication: A dual activation mechanism for Myb-responsive genes in myelomonocytic cells
Issued Date
2008-03-01
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ISSN
10799796
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2-s2.0-38949164007
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Mahidol University
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SCOPUS
Bibliographic Citation
Blood Cells, Molecules, and Diseases. Vol.40, No.2 (2008), 219-226
Suggested Citation
Vichanan Yamkamon, Olga Ivanova, Daniel Braas, Olesya Chayka, Pimpicha Patmasiriwat, Karl Heinz Klempnauer A dual activation mechanism for Myb-responsive genes in myelomonocytic cells. Blood Cells, Molecules, and Diseases. Vol.40, No.2 (2008), 219-226. doi:10.1016/j.bcmd.2007.08.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18959
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Title
A dual activation mechanism for Myb-responsive genes in myelomonocytic cells
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Abstract
The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biological effects by deregulating the expression of specific target genes. Here we have used DNaseI hypersensitive site mapping and reporter gene assays to study the activation of three Myb target genes - mim-1, the lysozyme gene and the C/EBPβ gene - all of which are activated by Myb in myelomonocytic cells but not in other hematopoietic lineages. We have found that these genes are activated by Myb via more than one cis-regulatory region. Our data suggest that all three genes are activated by Myb by dual mechanisms involving the promoters as well as enhancers. Using a cell line that expresses an estrogen-inducible v-Myb/estrogen receptor fusion protein we have also determined the effect of Myb on the expression of the C/EBPα gene. Our results show that C/EBPα expression is down-regulated by v-Myb. Thus, v-Myb affects the expression of two C/EBP family members in opposite directions. © 2007 Elsevier Inc. All rights reserved.