Publication: Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation
Issued Date
2014-01-01
Resource Type
ISSN
14661861
09629351
09629351
Other identifier(s)
2-s2.0-84904605564
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Mahidol University
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SCOPUS
Bibliographic Citation
Mediators of Inflammation. Vol.2014, (2014)
Suggested Citation
Salunya Tancharoen, Tassanee Tengrungsun, Theeralaksna Suddhasthira, Kiyoshi Kikuchi, Nuttavun Vechvongvan, Masayuki Tokuda, Ikuro Maruyama Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation. Mediators of Inflammation. Vol.2014, (2014). doi:10.1155/2014/754069 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33460
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Title
Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation
Abstract
High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular and inflammatory diseases. The aim of this study was to examine whether RAGE and HMGB1 are involved in the pathogenesis of pulpitis and investigate the effect of Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on RAGE and HMGB1 expression in odontoblast-like cells (OLC-1). RAGE and HMGB1 expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Upregulated expression of RAGE was observed in odontoblasts, stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cells in human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-B activation. These findings suggest that RAGE and HMGB1 play an important role in the pulpal immune response to oral bacterial infection. © 2014 Salunya Tancharoen et al.