Publication: Protocol for a facile multiplex PCR for multi-antimicrobial resistance and gonococcus detection
Issued Date
2012-08-15
Resource Type
ISSN
10643745
Other identifier(s)
2-s2.0-84864858382
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in Molecular Biology. Vol.903, (2012), 329-341
Suggested Citation
Ratana Lawung, Rungrot Cherdtrakulkiat, Virapong Prachayasittikul Protocol for a facile multiplex PCR for multi-antimicrobial resistance and gonococcus detection. Methods in Molecular Biology. Vol.903, (2012), 329-341. doi:10.1007/978-1-61779-937-2_23 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13635
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Protocol for a facile multiplex PCR for multi-antimicrobial resistance and gonococcus detection
Other Contributor(s)
Abstract
Gonorrhea is a continuing problem worldwide particularly in terms of the spread of multiple drug resistance. We have successfully developed an efficient PCR method for the simultaneous identification of gonococci and detection of the antimicrobial-resistant profile. By this method, penicillinase-producing Neisseria gonorrhoeae (PPNG), high-level tetracycline-resistant N. gonorrhoeae (TRNG), and ciprofloxacin-resistant N. gonorrhoeae (CRNG) can be clearly identified. Moreover, the plasmid-types of penicillin and tetracycline resistance are also characterized. The method has 100 % sensitivity and specificity. It is also time-and labor-saving compared to the conventional method. Thus, the procedure is suitable for epidemiological surveillance. © 2012 Springer Science+Business Media New York.