Publication: Gene expression and promoter characterization of heat-shock protein 90B gene (HSP90B) in the model unicellular green alga Chlamydomonas reinhardtii
Issued Date
2018-07-01
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ISSN
18732259
01689452
01689452
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2-s2.0-85046372480
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Science. Vol.272, (2018), 107-116
Suggested Citation
Somchoke Traewachiwiphak, Chotika Yokthongwattana, Parthompong Ves-Urai, Varodom Charoensawan, Kittisak Yokthongwattana Gene expression and promoter characterization of heat-shock protein 90B gene (HSP90B) in the model unicellular green alga Chlamydomonas reinhardtii. Plant Science. Vol.272, (2018), 107-116. doi:10.1016/j.plantsci.2018.04.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/44721
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Title
Gene expression and promoter characterization of heat-shock protein 90B gene (HSP90B) in the model unicellular green alga Chlamydomonas reinhardtii
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Abstract
© 2018 Elsevier B.V. Molecular chaperones or heat shock proteins are a large protein family with important functions in every cellular organism. Among all types of the heat shock proteins, information on the ER-localized HSP90 protein (HSP90B) and its encoding gene is relatively scarce in the literature, especially in photosynthetic organisms. In this study, expression profiles as well as promoter sequence of the HSP90B gene were investigated in the model green alga Chlamydomonas reinhardtii. We have found that HSP90B is strongly induced by heat and ER stresses, while other short-term exposure to abiotic stresses, such as salinity, dark-to-light transition or light stress does not appear to affect the expression. Promoter truncation analysis as well as chromatin immunoprecipitation using the antibodies recognizing histone H3 and acetylated histone H3, revealed a putative core constitutive promoter sequence between −1 to −253 bp from the transcription start site. Our results also suggested that the nucleotides upstream of the core promoter may contain repressive elements such as putative repressor binding site(s).