Publication: Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide
Issued Date
2014-01-01
Resource Type
ISSN
10985514
0022538X
0022538X
Other identifier(s)
2-s2.0-84904878889
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virology. Vol.88, No.16 (2014), 9406-9417
Suggested Citation
S. Moses Dennison, Kara M. Anasti, Frederick H. Jaeger, Shelley M. Stewart, Justin Pollara, Pinghuang Liu, Erika L. Kunz, Ruijun Zhang, Nathan Vandergrift, Sallie Permar, Guido Ferrari, Georgia D. Tomaras, Mattia Bonsignori, Nelson L. Michael, Jerome H. Kim, Jaranit Kaewkungwal, Sorachai Nitayaphan, Punnee Pitisuttithum, Supachai Rerks-Ngarm, Hua Xin Liao, Barton F. Haynes, S. Munir Alam Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide. Journal of Virology. Vol.88, No.16 (2014), 9406-9417. doi:10.1128/JVI.01031-14 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34052
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Title
Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide
Author(s)
S. Moses Dennison
Kara M. Anasti
Frederick H. Jaeger
Shelley M. Stewart
Justin Pollara
Pinghuang Liu
Erika L. Kunz
Ruijun Zhang
Nathan Vandergrift
Sallie Permar
Guido Ferrari
Georgia D. Tomaras
Mattia Bonsignori
Nelson L. Michael
Jerome H. Kim
Jaranit Kaewkungwal
Sorachai Nitayaphan
Punnee Pitisuttithum
Supachai Rerks-Ngarm
Hua Xin Liao
Barton F. Haynes
S. Munir Alam
Kara M. Anasti
Frederick H. Jaeger
Shelley M. Stewart
Justin Pollara
Pinghuang Liu
Erika L. Kunz
Ruijun Zhang
Nathan Vandergrift
Sallie Permar
Guido Ferrari
Georgia D. Tomaras
Mattia Bonsignori
Nelson L. Michael
Jerome H. Kim
Jaranit Kaewkungwal
Sorachai Nitayaphan
Punnee Pitisuttithum
Supachai Rerks-Ngarm
Hua Xin Liao
Barton F. Haynes
S. Munir Alam
Abstract
Mucosal epithelial cell surface galactosylceramide (Galcer) has been postulated to be a receptor for HIV-1 envelope (Env) interactions with mucosal epithelial cells. Disruption of the HIV-1 Env interaction with such alternate receptors could be one strategy to prevent HIV-1 entry through the mucosal barrier. To study antibody modulation of HIV-1 Env-Galcer interactions, we used Galcer-containing liposomes to assess whether natural- and vaccine-induced monoclonal antibodies can block HIV-1 Env binding to Galcer. HIV-1 Env gp140 proteins bound to Galcer liposomes with KdS (dissociation constants) in the nanomolar range. Several HIV-1 ALVAC/AIDSVAX vaccinee-derived monoclonal antibodies (MAbs) specific for the gp120 first constant (C1) region blocked Galcer binding of a transmitted/founder HIV-1 Env gp140. Among the C1- specific MAbs that showed Galcer blocking, the antibody-dependent cellular cytotoxicity-mediating CH38 IgG and its natural IgA isotype were the most potent blocking antibodies. C1-specific IgG monoclonal antibodies that blocked Env binding to Galcer induced upregulation of the gp120 CD4-inducible (CD4i) epitope bound by MAb 17B, demonstrating that a conformational change in gp120 may be required for Galcer blocking. However, the MAb 17B itself did not block Env-Galcer binding, suggesting that the C1 antibody-induced gp120 conformational changes resulted in alteration in a Galcer binding site distant from the CD4i 17B MAb binding site. © 2014, American Society for Microbiology.