Publication: Amelioration of intestinal reperfusion injury by moderate hypothermia is associated with serum sICAM-1 levels
Issued Date
2006-01-01
Resource Type
ISSN
10958673
00224804
00224804
Other identifier(s)
2-s2.0-29744466875
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Surgical Research. Vol.130, No.1 (2006), 152-157
Suggested Citation
Paisarn Vejchapipat, Nuchanan Leawhiran, Sopee Poomsawat, Apiradee Theamboonlers, Soottiporn Chittmittrapap, Yong Poovorawan Amelioration of intestinal reperfusion injury by moderate hypothermia is associated with serum sICAM-1 levels. Journal of Surgical Research. Vol.130, No.1 (2006), 152-157. doi:10.1016/j.jss.2005.07.034 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23858
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Title
Amelioration of intestinal reperfusion injury by moderate hypothermia is associated with serum sICAM-1 levels
Abstract
Background. The aim of this study was to investigate the effects of moderate hypothermia on various serum markers involving in inflammation after intestinal ischemia-reperfusion (IR). Materials and methods. The model of 30 min intestinal ischemia +90 min reperfusion was used. Three groups of rats were studied, n = 7-8 per group: 1) sham at normothermia, 36.5 to 37.5°C; 2) IR at normothermia and; 3) IR at moderate hypothermia, 32 to 33°C. Serum levels of TNF-α, lipopolysaccharide-inducible CXC chemokine (LIX), and soluble intercellular adhesion molecule-1 (sICAM-1) were determined using ELISA technique. Histological features of terminal ileum were also graded. Results. Intestinal IR at normothermia caused remarkable tissue injury together with an elevation in serum TNF-α, LIX, and sICAM-1 levels. Moderate hypothermia significantly decreased the degree of mucosal damage and attenuated the elevation of serum sICAM-1 levels. However, there were no significant differences in serum TNF-α and LIX levels between IR at normothermia and IR at hypothermia. Conclusions. Intestinal IR at normothermia induces the elevation of serum TNF-α, LIX, and sICAM-1 levels. Moderate hypothermia protects the small intestine from reperfusion injury. This beneficial effect is associated with serum sICAM-1 levels but not with serum TNF-α and LIX levels. We speculate that one of the mechanisms, by which hypothermia blunts the tissue injury, is at the step of firm adhesion between leukocytes and endothelial cells. © 2006 Elsevier Inc. All rights reserved.