Publication: Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis
Issued Date
2014-03-01
Resource Type
ISSN
10959947
10504648
10504648
Other identifier(s)
2-s2.0-84893472820
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Mahidol University
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SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.37, No.1 (2014), 46-52
Suggested Citation
Pakkakul Sangsuriya, Kornsunee Phiwsaiya, Benjamart Pratoomthai, Thanawat Sriphaijit, Piti Amparyup, Boonsirm Withyachumnarnkul, Saengchan Senapin Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis. Fish and Shellfish Immunology. Vol.37, No.1 (2014), 46-52. doi:10.1016/j.fsi.2014.01.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33050
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Title
Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis
Abstract
A novel G-protein pathway suppressor 2 (GPS2) has been identified from hemocytes of the whiteleg shrimp Penaeus vannamei (Pv) and appears to play a role in ecdysis. The full-length of PvGPS2 cDNA consisted of a 1230-bp open reading frame, encoding 409 deduced amino acids with significant sequence homology to GPS2 sequences of crustaceans and insects. RT-PCR revealed that PvGPS2 was expressed in all P.vannamei tissues examined, but that expression was molt stage specific in eyestalk tissue. Relative expression was higher in the period before molting (i.e., intermolt and pre-molt stages) than in the post-molt stage. When double-stranded RNA (dsRNA)-mediated RNA interference was employed to inhibit PvGPS2 formation in shrimp, it led to significant mortality due to unsuccessful separation of new cuticle from old cuticle (exuvial entrapment) during ecdysis. © 2014 Elsevier Ltd.